2026-06-14T03:37:43Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/173472024-09-21T20:52:51Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Guadix, Juan Antonio
author
Orlova, Valeria V
author
Giacomelli, Elisa
author
Bellin, Milena
author
Ribeiro, Marcelo C
author
Mummery, Christine L
author
Pérez-Pomares, José M
author
Passier, Robert
2024-01-23T20:13:19Z
2024-01-23T20:13:19Z
2017-11-22
10.1016/j.stemcr.2017.10.023
2213-6711
Stem cell reports
http://hdl.handle.net/10668/11845
29173898
http://hdl.handle.net/20.500.12105/17347
Human pluripotent stem cells (hPSCs) are widely used to study cardiovascular cell differentiation and function. Here, we induced differentiation of hPSCs (both embryonic and induced) to proepicardial/epicardial progenitor cells that cover the heart during development. Addition of retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted expression of the mesodermal marker PDGFRα, upregulated characteristic (pro)epicardial progenitor cell genes, and downregulated transcription of myocardial genes. We confirmed the (pro)epicardial-like properties of these cells using in vitro co-culture assays and in ovo grafting of hPSC-epicardial cells into chick embryos. Our data show that RA + BMP4-treated hPSCs differentiate into (pro)epicardial-like cells displaying functional properties (adhesion and spreading over the myocardium) of their in vivo counterpart. The results extend evidence that hPSCs are an excellent model to study (pro)epicardial differentiation into cardiovascular cells in human development and evaluate their potential for cardiac regeneration.
eng
cardiovascular
differentiation
human pluripotent stem cells
proepicardium
progenitor cells
Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
research article