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oai:repisalud.isciii.es:20.500.12105/173472024-09-21T20:52:51Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Guadix, Juan Antonio author Orlova, Valeria V author Giacomelli, Elisa author Bellin, Milena author Ribeiro, Marcelo C author Mummery, Christine L author Pérez-Pomares, José M author Passier, Robert author 2017-11-22 Human pluripotent stem cells (hPSCs) are widely used to study cardiovascular cell differentiation and function. Here, we induced differentiation of hPSCs (both embryonic and induced) to proepicardial/epicardial progenitor cells that cover the heart during development. Addition of retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted expression of the mesodermal marker PDGFRα, upregulated characteristic (pro)epicardial progenitor cell genes, and downregulated transcription of myocardial genes. We confirmed the (pro)epicardial-like properties of these cells using in vitro co-culture assays and in ovo grafting of hPSC-epicardial cells into chick embryos. Our data show that RA + BMP4-treated hPSCs differentiate into (pro)epicardial-like cells displaying functional properties (adhesion and spreading over the myocardium) of their in vivo counterpart. The results extend evidence that hPSCs are an excellent model to study (pro)epicardial differentiation into cardiovascular cells in human development and evaluate their potential for cardiac regeneration. 10.1016/j.stemcr.2017.10.023 2213-6711 Stem cell reports http://hdl.handle.net/10668/11845 29173898 http://hdl.handle.net/20.500.12105/17347 cardiovascular differentiation human pluripotent stem cells proepicardium progenitor cells Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.