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00925njm 22002777a 4500 dc Gutiérrez-de-Juan, Virginia author López de Davalillo, Sergio author Fernández-Ramos, David author Barbier-Torres, Lucía author Zubiete-Franco, Imanol author Fernández-Tussy, Pablo author Simon, Jorge author Lopitz-Otsoa, Fernando author de Las Heras, Javier author Iruzubieta, Paula author Arias-Loste, María Teresa author Villa, Erica author Crespo, Javier author Andrade, Raúl author Lucena, M Isabel author Varela-Rey, Marta author Lu, Shelly C author Mato, José M author Delgado, Teresa Cardoso author Martínez-Chantar, María-Luz author 2017-03-20 Hyperammonemia is a metabolic condition characterized by elevated levels of ammonia and a common event in acute liver injury/failure and chronic liver disease. Even though hepatic ammonia levels are potential predictive factors of patient outcome, easy and inexpensive methods aiming at the detection of liver ammonia accumulation in the clinical setting remain unavailable. Thus, herein we have developed a morphological method, based on the utilization of Nessler´s reagent, to accurately and precisely detect the accumulation of ammonia in biological tissue. We have validated our method against a commercially available kit in mouse tissue samples and, by using this modified method, we have confirmed the hepatic accumulation of ammonia in clinical and animal models of acute and chronic advanced liver injury as well as in the progression of fatty liver disease. Overall, we propose a morphological method for ammonia detection in liver that correlates well with the degree of liver disease severity and therefore can be potentially used to predict patient outcome. 10.1371/journal.pone.0173914 1932-6203 PloS one http://hdl.handle.net/10668/10978 28319158 http://hdl.handle.net/20.500.12105/17299 A morphological method for ammonia detection in liver.