2026-04-29T22:51:34Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/172892024-11-28T15:18:26Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Sanchez-Marin, Laura
author
Pavón, Francisco-Javier
author
Decara, Juan
author
Suarez, Juan
author
Gavito, Ana
author
Castilla-Ortega, Estela
author
Rodriguez de Fonseca, Fernando
author
Serrano, Antonia
authoraffiliation
Unidad de Gestion Clinica de Salud Mental, Instituto de Investigacion Biomedica de Malaga, Hospital Regional Universitario de Malaga, Universidad de Malaga, Malaga, Spain.
2024-01-23T20:12:27Z
2024-01-23T20:12:27Z
2017-02-07
10.3389/fnbeh.2017.00015
1662-5153
Frontiers in Behavioral Neuroscience
http://hdl.handle.net/10668/2669
28223925
http://hdl.handle.net/20.500.12105/17289
Intermittent alcohol exposure is a common pattern of adolescent alcohol use that can lead to binge drinking episodes. Alcohol use is known to modulate the endocannabinoid system (ECS), which is involved in neuronal communication, neuroplasticity, neuroinflammation and behavior. Adolescent male Wistar rats were exposed to 4-week intermittent alcohol intoxication (3 g/kg injections for 4 days/week) or saline (N = 12 per group). After alcohol deprivation, adult rats were assessed for emotionality and cognition and the gene expression of the ECS and other factors related to behavior and neuroinflammation was examined in the brain. Alcohol-exposed rats exhibited anxiogenic-like responses and impaired recognition memory but no motor alterations. There were brain region-dependent changes in the mRNA levels of the ECS and molecular signals compared with control rats. Thus, overall, alcohol-exposed rats expressed higher mRNA levels of endocannabinoid synthetic enzymes (N-acyl-phosphatidylethanolamine phospholipase D and diacylglycerol lipases) in the medial-prefrontal cortex (mPFC) but lower mRNA levels in the amygdala. Furthermore, we observed lower mRNA levels of receptors CB1 CB2 and peroxisome proliferator-activated receptor-α in the striatum. Regarding neuropeptide signaling, alcohol-exposed rats displayed lower mRNA levels of the neuropeptide Y signaling, particularly NPY receptor-2, in the amygdala and hippocampus and higher mRNA levels of corticotropin-releasing factor in the hippocampus. Additionally, we observed changes of several neuroinflammation-related factors. Whereas, the mRNA levels of toll-like receptor-4, tumor necrosis factor-α, cyclooxygenase-2 and glial fibrillary acidic protein were significantly increased in the mPFC, the mRNA levels of cyclooxygenase-2 and glial fibrillary acidic protein were decreased in the striatum and hippocampus. However, nuclear factor-κβ mRNA levels were lower in the mPFC and striatum and allograft inflammatory factor-1 levels were differentially expressed in the amygdala and hippocampus. In conclusion, rats exposed to adolescent intermittent alcohol displayed anxiety-like behavior and cognitive deficits in adulthood and these alterations were accompanied by brain region-dependent changes in the gene expression of the ECS and other signals associated with neuroinflammation and behavior. An intermittent adolescent alcohol exposure has behavioral and molecular consequences in the adult brain, which might be linked to higher vulnerability to addictive behaviors and psychopathologies.
eng
Rats
Intermittent alcohol
Adolescence
Endocannabinoid system
Neuroinflammation
Anxiety
Brain
Recognition memory
Adolescente
Intoxicación alcohólica
Aloinjertos
Amígdala del cerebelo
Animales
Ansiedad
Conducta adictiva
Borrachera
Cognición
Hormona liberadora de corticotropina
Ciclooxigenasa 2
Endocannabinoides
Etanol
Proteína ácida fibrilar de la glía
Hipocampo
Humanos
Lipoproteína lipasa
Plasticidad neuronal
Neuropéptido Y
PPAR alfa
Fosfatidiletanolaminas
Fosfolipasa D
Corteza prefrontal
ARN mensajero
Ratas wistar
Receptor cannabinoide CB1
Receptor cannabinoide CB2
Receptor Toll-Like 4
Factor de necrosis tumoral alfa
Consumo de alcohol en menores
Effects of Intermittent Alcohol Exposure on Emotion and Cognition: A Potential Role for the Endogenous Cannabinoid System and Neuroinflammation
research article