2026-04-27T03:42:44Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/172802024-09-21T21:01:25Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Rodriguez, Maria J author Aranda, Ana author Fernandez, Tahia D author Cubells-Baeza, Nuria author Torres, Maria J author Gomez, Francisca author Palomares, Francisca author Perkins, James R author Rojo, Javier author Diaz-Perales, Araceli author Mayorga, Cristobalina author 2017-01-13 Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model of peach anaphylaxis by sensitising mice with Pru p 3 in combination with lipopolysaccharide (LPS) as an adjuvant. Four groups of mice were sensitised intranasally: untreated; treated with Pru p 3; treated with LPS; treated with Pru p 3 + LPS. After sensitisation mice were intraperitoneally challenged with Pru p 3 and in vivo and in vitro parameters were evaluated. Only mice in the Pru p 3 + LPS group showed anaphylaxis symptoms, including a decrease in temperature. Determination of in vitro parameters showed a Th2 response with an increase of Pru p 3-specific IgE and IgG1. Moreover, at the cellular level, we found increased levels of IgE and IgG1 secreting Pru p 3-specific cells and a proliferative CD4+ T-cell response. These results demonstrate that Pru p 3-specific anaphylaxis can be generated after nasal sensitisation to Pru p 3 in combination with LPS. This is a promising model for evaluating food allergy immunotherapies. http://hdl.handle.net/10668/10771 http://hdl.handle.net/20.500.12105/17280 28084419 10.1038/srep40449 2045-2322 Scientific reports LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model.