2026-08-29T14:12:18Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/172542025-05-08T14:53:31Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16973

00925njm 22002777a 4500 dc Mata, Elena author Tarancón, Raquel author Guerrero, Claudia author Moreo, Eduardo author Moreau, Flavie author Uranga, Santiago author Gomez, Ana Belen author Marinova, Dessislava author Domenech Lucas, Mirian author Gonzalez-Camacho, Fernando author Monzón, Marta author Badiola, Juan author Domínguez-Andrés, Jorge author Yuste, Jose Enrique author Anel, Alberto author Peixoto, Antonio author Martin, Carlos author Aguilo, Nacho author 2021-09-24 Bacillus Calmette-Guerin (BCG) is an attenuated bacterial vaccine used to protect against Mycobacterium tuberculosis (Mtb) in regions where infections are highly prevalent. BCG is currently delivered by the intradermal route, but alternative routes of administration are of great interest, including intrapulmonary delivery to more closely mimic respiratory Mtb infection. In this study, mice subjected to pulmonary delivery of green fluorescent protein–tagged strains of virulent (Mtb) and attenuated (BCG) mycobacteria were studied to better characterize infected lung cell subsets. Profound differences in dissemination patterns were detected between Mtb and BCG, with a strong tendency of Mtb to disseminate from alveolar macrophages (AMs) to other myeloid subsets, mainly neutrophils and recruited macrophages. BCG mostly remained in AMs, which promoted their activation. These preactivated macrophages were highly efficient in containing Mtb bacilli upon challenge and disrupting early bacterial dissemination, which suggests a potential mechanism of protection associated with pulmonary BCG vaccination. Respiratory BCG also protected mice against a lethal Streptococcus pneumoniae challenge, suggesting that BCG-induced innate activation could confer heterologous protection against respiratory pathogens different from Mtb. BCG drove long-term activation of AMs, even after vaccine clearance, and these AMs reacted efficiently upon subsequent challenge. These results suggest the generation of a trained innate memory-like response in AMs induced by pulmonary BCG vaccination. Sci Immunol. 2021 Sep 24;6(63):eabc2934. 10.1126/sciimmunol.abc2934 2470-9468 Science immunology 34559551 http://hdl.handle.net/20.500.12105/17254 Pulmonary BCG induces lung-resident macrophage activation and confers long-term protection against tuberculosis