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oai:repisalud.isciii.es:20.500.12105/172442024-11-28T15:52:37Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16938

00925njm 22002777a 4500 dc Carrasco-Antón, Nerea author Ibarra-Meneses, Ana Victoria author Carrillo, Eugenia author Fernández-Ruiz, Mario author Hernández-Jiménez, Pilar author Aguado, José María author Moreno, Javier author López-Medrano, Francisco author 2022-04 Objective: Sensitive and less laborious assays are needed to detect asymptomatic Leishmania among solid organ transplant (SOT) recipients. Using SLA-stimulated plasma from SOT recipients living where an outbreak of Leishmania infantum occurred, we examined potential biomarkers to identify asymptomatic Leishmania infections. Methods: Concentrations of cytokines/chemokines in plasma from whole blood stimulated with specific Leishmania antigen (SLA) were compared against infection status as determined by a currently used cell proliferation assay. Results: Twenty-six percent (13/50) of the SOT recipients had a cell proliferation assay (CPA) indicating asymptomatic infection, and showed higher processed plasma C-X-C motif chemokine ligand 10 (CXCL10 or IP-10) concentrations than did non-infected subjects (median 2272.0 pg/ml [IQR 1570-2772] vs. 18.2 pg/ml [IQR 1-150.1]; p<0.0001). CXCL10 showed a sensitivity of 93% and a specificity of 95% compared to CPA. In addition, we demonstrated that the number of asymptomatic infections detected using CXCL10, decreased with distance from a park at the center of the mentioned outbreak. Conclusion: CXCL10 in plasma from SLA-stimulated blood could be a robust biomarker of asymptomatic L. infantum infection in solid organ transplant recipients. J Infect. 2022 Apr;84(4):573-578. 10.1016/j.jinf.2022.01.029 1532-2742 The Journal of infection 35101535 http://hdl.handle.net/20.500.12105/17244 SSolid-organ transplant recipients Leishmania Leishmaniasis Biomarkers Asymptomatic infection IP-10 CXCL10 An exploratory analysis of C-X-C motif chemokine ligand 10 as a new biomarker of asymptomatic Leishmania infantum infection in solid-organ transplant recipients