2026-05-17T01:18:37Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/172022024-09-21T13:24:00Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Sá da Bandeira, Diana author Kilpatrick, Alastair Morris author Marques, Madalena author Gomez-Salazar, Mario author Ventura, Telma author Gonzalez, Zaniah Nashira author Stefancova, Dorota author Rossi, Fiona author Vermeren, Matthieu author Vink, Chris Sebastiaan author Beltran, Mariana author Henderson, Neil Cowan author Jung, Bongnam author van der Linden, Reinier author van de Werken, Harmen Jan George author van Ijcken, Wilfred F J author Betsholtz, Christer author Forbes, Stuart John author Cuervo, Henar author Crisan, Mihaela author 2022-07-19 Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro. Cell Rep. 2022 Jul 19;40(3):111114. 10.1016/j.celrep.2022.111114 2211-1247 Cell reports 35858557 http://hdl.handle.net/20.500.12105/17202 PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny.