2026-06-14T02:27:58Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/171792024-11-28T18:37:17Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19608col_20.500.12105_19609col_20.500.12105_16963
Repisalud
author
Gavilán, Ana M
author
Perán-Ramos, Paula
author
Sanz, Juan Carlos
author
García-Comas, Luis
author
Pérez-Abeledo, Marta
author
Castellanos-Nadal, Ana Maria
author
Berciano, Jose Miguel
author
Lopez-Perea, Noemi
author
Masa-Calles, Josefa
author
Echevarria, Juan Emilio
author
Fernandez-Garcia, Aurora
funder
Centro de Investigación Biomédica en Red - CIBERESP (Epidemiología y Salud Pública)
funder
Instituto de Salud Carlos III
2024-01-16T13:02:11Z
2024-01-16T13:02:11Z
2023-12-13
Viruses. 2023 Dec 13;15(12):2420.
10.3390/v15122420
1999-4915
Viruses
38140661
http://hdl.handle.net/20.500.12105/17179
Mumps is a vaccine-preventable disease caused by the mumps virus (MuV). However, MuV has re-emerged in many countries with high vaccine coverage. The World Health Organization (WHO) recommends molecular surveillance based on sequencing of the small hydrophobic (SH) gene. Additionally, the combined use of SH and non-coding regions (NCR) has been described in different studies, proving to be a useful complement marker to discriminate general patterns of circulation at national and international levels. The aim of this work is to test local-level usefulness of the combination of SH and MF-NCR sequencing in tracing hidden transmission clusters and chains during the last epidemic wave (2015-2020) in Spain. A database with 903 cases from the Autonomous Community of Madrid was generated by the integration of microbiological and epidemiological data. Of these, 453 representative cases were genotyped. Eight different SH variants and thirty-four SH haplotypes were detected. Local MuV circulation showed the same temporal pattern previously described at a national level. Only two of the thirteen previously identified outbreaks were caused by more than one variant/haplotype. Geographical representation of SH variants allowed the identification of several previously undetected clusters, which were analysed phylogenetically by the combination of SH and MF-NCR, in a total of 90 cases. MF-NCR was not able to improve the discrimination of geographical clusters based on SH sequencing, showing limited resolution for outbreak investigations.
eng
Laboratory surveillance
Molecular epidemiology
Genotyping
MF-NCR sequence
SH sequence
Mumps virus
Mumps
Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools
research article
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InvestigatingLocalPatternsMumpsVirus_2023.pdf.txt
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Correct- Supplem_table2_InvestigatingLocalPatternsMumpsVirus_2023.xlsx.txt