2026-06-14T02:29:19Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/171792024-11-28T18:37:17Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19608col_20.500.12105_19609col_20.500.12105_16963

00925njm 22002777a 4500 dc Gavilán, Ana M author Perán-Ramos, Paula author Sanz, Juan Carlos author García-Comas, Luis author Pérez-Abeledo, Marta author Castellanos-Nadal, Ana Maria author Berciano, Jose Miguel author Lopez-Perea, Noemi author Masa-Calles, Josefa author Echevarria, Juan Emilio author Fernandez-Garcia, Aurora author 2023-12-13 Mumps is a vaccine-preventable disease caused by the mumps virus (MuV). However, MuV has re-emerged in many countries with high vaccine coverage. The World Health Organization (WHO) recommends molecular surveillance based on sequencing of the small hydrophobic (SH) gene. Additionally, the combined use of SH and non-coding regions (NCR) has been described in different studies, proving to be a useful complement marker to discriminate general patterns of circulation at national and international levels. The aim of this work is to test local-level usefulness of the combination of SH and MF-NCR sequencing in tracing hidden transmission clusters and chains during the last epidemic wave (2015-2020) in Spain. A database with 903 cases from the Autonomous Community of Madrid was generated by the integration of microbiological and epidemiological data. Of these, 453 representative cases were genotyped. Eight different SH variants and thirty-four SH haplotypes were detected. Local MuV circulation showed the same temporal pattern previously described at a national level. Only two of the thirteen previously identified outbreaks were caused by more than one variant/haplotype. Geographical representation of SH variants allowed the identification of several previously undetected clusters, which were analysed phylogenetically by the combination of SH and MF-NCR, in a total of 90 cases. MF-NCR was not able to improve the discrimination of geographical clusters based on SH sequencing, showing limited resolution for outbreak investigations. Viruses. 2023 Dec 13;15(12):2420. 10.3390/v15122420 1999-4915 Viruses 38140661 http://hdl.handle.net/20.500.12105/17179 Laboratory surveillance Molecular epidemiology Genotyping MF-NCR sequence SH sequence Mumps virus Mumps Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools