2026-07-24T06:25:18Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/171632024-09-21T18:28:14Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author Ariza, Adriana author Mayorga, Cristobalina author Salas, María author Doña, Inmaculada author Martín-Serrano, Ángela author Pérez-Inestrosa, Ezequiel author Pérez-Sala, Dolores author Guzmán, Antonio E author Montañez, María I author Torres, María J 2024-01-16T12:16:37Z 2024-01-16T12:16:37Z 2016-10-12 10.1038/srep35113 2045-2322 Scientific reports http://hdl.handle.net/10668/10528 27731424 http://hdl.handle.net/20.500.12105/17163 The optimal recognition of penicillin determinants, including amoxicillin (AX), by specific IgE antibodies is widely believed to require covalent binding to a carrier molecule. The nature of the carrier and its contribution to the antigenic determinant is not well known. Here we aimed to evaluate the specific-IgE recognition of different AX-derived structures. We studied patients with immediate hypersensitivity reactions to AX, classified as selective or cross-reactors to penicillins. Competitive immunoassays were performed using AX itself, amoxicilloic acid, AX bound to butylamine (AXO-BA) or to human serum albumin (AXO-HSA) in the fluid phase, as inhibitors, and amoxicilloyl-poli-L-lysine (AXO-PLL) in the solid-phase. Two distinct patterns of AX recognition by IgE were found: Group A showed a higher recognition of AX itself and AX-modified components of low molecular weights, whilst Group B showed similar recognition of both unconjugated and conjugated AX. Amoxicilloic acid was poorly recognized in both groups, which reinforces the need for AX conjugation to a carrier for optimal recognition. Remarkably, IgE recognition in Group A (selective responders to AX) is influenced by the mode of binding and/or the nature of the carrier; whereas IgE in Group B (cross-responders to penicillins) recognizes AX independently of the nature of the carrier. eng The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams. research article