2026-07-23T23:02:14Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/171582024-11-28T15:40:42Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927col_20.500.12105_16980
Repisalud
author
Pavón, Francisco-Javier
author
Marco, Eva María
author
Vázquez, Mariam
author
Sánchez, Laura
author
Rivera, Patricia
author
Gavito, Ana
author
Mela, Virginia
author
Alén, Francisco
author
Decara, Juan
author
Suárez, Juan
author
Giné, Elena
author
López-Moreno, José Antonio
author
Chowen, Julie
author
Rodríguez-de-Fonseca, Fernando
author
Serrano, Antonia
author
Viveros, María Paz
authoraffiliation
[Pavón,FJ; Vázquez,M; Sánchez,L; Rivera,P; Gavito,A; Alén,F; Decara,J; Suárez ,J; Rodríguez-de-Fonseca,F; Serrano,A] Unidad Gestión Clínica de Salud Mental, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario de Málaga-Universidad de Málaga, Málaga, Spain. [Marco,EM; Mela,V; Viveros,MP] Departamento de Fisiología (Fisiología Animal II), Facultad de Biología, Universidad Complutense, Madrid, Spain. [Giné,E; López-Moreno,JA] Departamento de Biología Celular, Facultad de Psicología, Universidad Complutense, Madrid, Spain. [Chowen,J] Servicio de Pediatría y Endocrinología Pediátrica, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación Sanitaria del Hospital Universitario de La Princesa (IP), Madrid, Spain. Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (CIBERobn) Instituto de Salud Carlos III, Madrid, Spain.
2024-01-16T12:16:33Z
2024-01-16T12:16:33Z
2016-09-23
10.1371/journal.pone.0163752
1932-6203
PloS One
http://hdl.handle.net/10668/2525
27662369
http://hdl.handle.net/20.500.12105/17158
Intermittent alcohol exposure is a common pattern of alcohol consumption among adolescents and alcohol is known to modulate the expression of the endocannabinoid system (ECS), which is involved in metabolism and inflammation. However, it is unknown whether this pattern may have short-term consequences on the ECS in the spleen. To address this question, we examined the plasma concentrations of metabolic and inflammatory signals and the splenic ECS in early adult rats exposed to alcohol during adolescence. A 4-day drinking in the dark (DID) procedure for 4 weeks was used as a model of intermittent forced-alcohol administration (20%, v/v) in female and male Wistar rats, which were sacrificed 2 weeks after the last DID session. First, there was no liver damage or alterations in plasma metabolic parameters. However, certain plasma inflammatory signals were altered according to sex and alcohol exposition. Whereas fractalkine [chemokine (C-X3-C motif) ligand 1] was only affected by sex with lower concentration in male rats, there was an interaction between sex and alcohol exposure in the TNF-α and interleukin-6 concentrations and only female rats displayed changes. Regarding the mRNA and protein expression of the ECS, the receptors and endocannabinoid-synthesizing enzymes were found to be altered with area-specific expression patterns in the spleen. Overall, whereas the expression of the cannabinoid receptor CB1 and the nuclear peroxisome proliferator-activated receptor PPARα were lower in alcohol-exposed rats compared to control rats, the CB2 expression was higher. Additionally, the N-acyl-phosphatidylethanolamine-specific phospholipase D expression was high in female alcohol-exposed rats and low in male alcohol-exposed rats. In conclusion, intermittent alcohol consumption during adolescence may be sufficient to induce short-term changes in the expression of splenic endocannabinoid signaling-related proteins and plasma pro-inflammatory cytokines in young adult rats with a strong sexual dimorphism. The potential impact of these alterations in early adulthood remains to be elucidated.
eng
Consumo de bebidas alcohólicas
Animales
Quimiocina CX3CL1
Citocinas
Ingestión de líquidos
Endocannabinoides
Etanol
Femenino
Inflamación
Interleucina-6
Hígado
Masculino
PPAR alfa
Fosfatidiletanolaminas
Fosfolipasa D
ARN mensajero
Ratas
Ratas wistar
Receptor cannabinoide CB1
Bazo
Factor de necrosis tumoral alfa
Effects of Adolescent Intermittent Alcohol Exposure on the Expression of Endocannabinoid Signaling-Related Proteins in the Spleen of Young Adult Rats.
research article