2026-05-17T01:44:07Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/171412024-11-28T15:12:12Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Gentile, Adriana-Mariel
author
Lhamyani, Said
author
Coín-Aragüez, Leticia
author
Oliva-Olivera, Wilfredo
author
Zayed, Hatem
author
Vega-Rioja, Antonio
author
Monteseirin, Javier
author
Romero-Zerbo, Silvana-Yanina
author
Tinahones, Francisco-José
author
Bermúdez-Silva, Francisco-Javier
author
El Bekay, Rajaa
authoraffiliation
[Gentile,AM; Lhamyani,S] IBIMA, Universidad de Málaga, Málaga, España. [Coín-Aragüez,L; Oliva-Olivera,W; Tinahones,FJ; El Bekay,R] CIBER Pathophysiology of obesity and nutrition CB06/03, Carlos III Health Institute, Malaga, Spain, Laboratory of Biomedical Research, Virgen de la Victoria Clinical University Hospital, Málaga, Spain. [Zayed,H] Biomedical Sciences Program, Health Sciences Department, College of Arts and Sciences, Qatar University, Doha, Qatar. [Vega-Rioja,A; Monteseirin,J] Unidad de Gestión Clínica de Alergia Intercentros, Hospital Universitario Virgen Macarena, Sevilla, Spain. [Romero-Zerbo,SY; Bermudez-Silva,FJ] Unidad de Gestion Clínica Intercentros de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario de Málaga/Universidad de Málaga, Málaga, Spain. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Málaga, Spain. [Tinahones,FJ] Endocrinology Service, Virgen de la Victoria Clinical University Hospital, Malaga, Spain.
2024-01-16T12:16:15Z
2024-01-16T12:16:15Z
2016-06-15
10.1371/journal.pone.0157002
1932-6203
PloS One
http://hdl.handle.net/10668/2443
27304673
http://hdl.handle.net/20.500.12105/17141
Real-time or quantitative PCR (qPCR) is a useful technique that requires reliable reference genes for data normalization in gene expression analysis. Adipogenesis is among the biological processes suitable for this technique. The selection of adequate reference genes is essential for qPCR gene expression analysis of human Vascular Stromal Cells (hVSCs) during their differentiation into adipocytes. To the best of our knowledge, there are no studies validating reference genes for the analyses of visceral and subcutaneous adipose tissue hVSCs from subjects with different Body Mass Index (BMI) and Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index. The present study was undertaken to analyze this question. We first analyzed the stability of expression of five potential reference genes: CYC, GAPDH, RPL13A, EEF1A1, and 18S ribosomal RNA, during in vitro adipogenic differentiation, in samples from these types of patients. The expression of RPL13A and EEF1A1 was not affected by differentiation, thus being these genes the most stable candidates, while CYC, GAPDH, and 18S were not suitable for this sort of analysis. This work highlights that RPL13A and EEF1A1 are good candidates as reference genes for qPCR analysis of hVSCs differentiation into adipocytes from subjects with different BMI and HOMA-IR.
eng
Adipocitos
Adipogénesis
Procesos biológicos
Índice de masa corporal
Expresión génica
Humanos
Resistencia a la insulina
Reacción en cadena de la polimerasa en tiempo real
Células del estroma
Grasa subcutánea
RPL13A and EEF1A1 Are Suitable Reference Genes for qPCR during Adipocyte Differentiation of Vascular Stromal Cells from Patients with Different BMI and HOMA-IR.
research article