2026-07-22T20:06:09Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/171292024-02-09T16:13:05Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Pérez-Martín, Margarita
author
Rivera, Patricia
author
Blanco, Eduardo
author
Lorefice, Clara
author
Decara, Juan
author
Pavón, Francisco-Javier
author
Serrano, Antonia
author
Rodríguez de Fonseca, Fernando
author
Suárez, Juan
2024-01-16T12:16:03Z
2024-01-16T12:16:03Z
2016-03-09
10.3389/fnins.2016.00089
1662-4548
Frontiers in neuroscience
http://hdl.handle.net/10668/9945
27013951
http://hdl.handle.net/20.500.12105/17129
Peroxisome proliferator-activated receptor alpha (PPARα) ligands have been shown to modulate recovery after brain insults such as ischemia and irradiation by enhancing neurogenesis. In the present study, we investigated the effect of the genetic deletion of PPARα receptors on the proliferative rate of neural precursor cells (NPC) in the adult brain. The study was performed in aged Pparα(-/-) mice exposed to nutritional (treats) and environmental (games) enrichments for 20 days. We performed immunohistochemical analyses of cells containing the replicating cell DNA marker 5-bromo-2'-deoxyuridine (BrdU+) and the immature neuronal marker doublecortin (Dcx+) in the main neurogenic zones of the adult brain: subgranular zone of dentate gyrus (SGZ), subventricular zone of lateral ventricles (SVZ), and/or hypothalamus. Results indicated a reduction in the number of BrdU+ cells in the neurogenic zones analyzed as well as Dcx+ cells in the SGZ during aging (2, 6, and 18 months). Pparα deficiency alleviated the age-related reduction of NPC proliferation (BrdU+ cells) in the SVZ of the 18-months-old mice. While no genotype effect on NPC proliferation was detected in the SGZ during aging, an accentuated reduction in the number of Dcx+ cells was observed in the SGZ of the 6-months-old Pparα(-/-) mice. Exposing the 18-months-old mice to nutritional and environmental enrichments reversed the Pparα(-/-)-induced impairment of NPC proliferation in the neurogenic zones analyzed. The enriched environment did not modify the number of SGZ Dcx+ cells in the 18 months old Pparα(-/-) mice. These results identify PPARα receptors as a potential target to counteract the naturally observed decline in adult NPC proliferation associated with aging and impoverished environments.
eng
PPARα
aging
environment
hippocampus
neurogenesis
subventricular zone
Environmental Enrichment, Age, and PPARα Interact to Regulate Proliferation in Neurogenic Niches.
research article