2026-04-29T12:24:09Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/171082024-11-28T15:35:03Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Oliva-Olivera, Wilfredo author Coín-Aragüez, Leticia author Salas, Julián author Lhamyani, Said author Gentile, Adriana-Mariel author Sarria García, Esteban author Hmadcha, Abdelkrim author Zayed, Hatem author Vega-Rioja, Antonio author Tinahones, Francisco J author El Bekay, Rajaa author 2015-12-10 OBJECTIVE: Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells. MATERIAL AND METHODS: We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects. RESULTS: TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs. CONCLUSIONS: This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine. 10.1371/journal.pone.0144401 1932-6203 PloS One http://hdl.handle.net/10668/2403 26657132 http://hdl.handle.net/20.500.12105/17108 Tejido adiposo Anciano Diferenciación celular Adipogénesis Proliferación de la célula Ensayo de unidades formadoras de colonias Proteínas de unión a ácidos Ggrasos Citometría de flujo Expresión génica Inmunofenotipificación Células del estroma mesenquimatoso Mediana edad Isquemia miocárdica Osteogénesis Medicina regenerativa Reacción en cadena de la polimerasa de transcriptasa inversa Proteína 1 de unión a los elementos reguladores de esteroles Grasa subcutánea Timo Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells.