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oai:repisalud.isciii.es:20.500.12105/171062024-11-28T14:43:18Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author García-Fuentes, Eduardo author Santiago-Fernández, Concepción author Gutiérrez-Repiso, Carolina author Mayas, María D author Oliva-Olivera, Wilfredo author Coín-Aragüez, Leticia author Alcaide, Juan author Ocaña-Wilhelmi, Luis author Vendrell, Joan author Tinahones, Francisco J author Garrido-Sánchez, Lourdes authoraffiliation [García-Fuentes,E; Gutiérrez-Repiso,C] Department of Endocrinology and Nutrition, Institute of Biomedical Research of Malaga (IBIMA), Regional University Hospital, Malaga, Spain. [García-Fuentes,E; Oliva-Olivera,W; Coín-Aragüez, L; Alcaide,J; Tinahones,FJ; Garrido-Sánchez,L] CIBEROBN, Institute of Health Carlos III, Malaga, Spain. [Santiago-Fernández,C; Oliva-Olivera,W; Coín-Aragüez,L; Alcaide,J; Tinahones,FJ; Garrido-Sánchez,L] Department of Endocrinology and Nutrition, Institute of Biomedical Research of Malaga (IBIMA), Virgen de la Victoria Clinical University Hospital, Malaga, Spain. [Mayas,MD] Department of Physiology, University of Jaen, Jaén, Spain. [Ocaña-Wilhelmi,L] Department of Surgery, Institute of Biomedical Research of Malaga (IBIMA), Virgen de la Victoria Clinical University Hospital, Malaga, Spain. [Vendrell,J] CIBERDEM, Institute of Health Carlos III, Tarragona, Spain. Department of Endocrinology and Nutrition, Joan XXIII University Hospital, Pere Virgili Institute, Rovira i Virgili University, Tarragona, Spain. [García-Fuentes,E] Laboratorio de Investigación, Hospital Civil, Málaga, Spain. 2024-01-15T18:17:53Z 2024-01-15T18:17:53Z 2015-11-30 10.1186/s12967-015-0732-5 1479-5876 Journal of Translational Medicine http://hdl.handle.net/10668/2449 26619907 http://hdl.handle.net/20.500.12105/17106 BACKGROUND: A key role for HIF-1α in the promotion and maintenance of dietary obesity has been proposed. We analyzed the association between hypoxia and de novo lipogenesis in human adipose tissue. METHODS: We studied HIF-1α mRNA and protein expression in fasting status in visceral adipose tissue (VAT) from non-obese and morbidly obese subjects, and in VAT from wild-type and ob/ob C57BL6J mice in both fasting and feeding status. We also analyzed the effect of hypoxia on the VAT mRNA expression of genes involved in lipogenesis. RESULTS: HIF-1α was increased in VAT from morbidly obese subjects. In fasting status, C57BL6J ob/ob mice had a higher VAT HIF-1α mRNA expression than C57BL6J wild-type mice. In feeding status, VAT HIF-1α mRNA expression significantly increased in C57BL6J wild-type, but not in C57BL6J ob/ob mice. In humans, HIF-1α mRNA expression correlated positively with body mass index and insulin resistance. VAT HIF-1α mRNA expression correlated negatively with ACC1, PDHB and SIRT3 mRNA expression, and positively with PPAR-γ. VAT explants incubated in hypoxia showed reduced SIRT3 and increased PPAR-γ, SREBP-1c, ACLY, ACC1 and FASN mRNA expression. CONCLUSIONS: Morbidly obese subjects have a higher level of VAT HIF-1α. Postprandial status is associated with an increase in HIF-1α mRNA expression in C57BL6J wild-type mice. Hypoxia alters the mRNA expression of genes involved in de novo lipogenesis in human VAT. eng Adipogénesis Tejido adiposo Índice de masa corporal Dieta Ayuno Factor 1 inducible por hipoxia Resistencia a la insulina Grasa intraabdominal Lipogénesis Ratones Humanos Obesidad mórbida PPAR gamma ARN mensajero Proteína 1 de unión a elementos reguladores de esteroles Hypoxia is associated with a lower expression of genes involved in lipogenesis in visceral adipose tissue. research article