2026-04-30T03:51:11Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/171062024-11-28T14:43:18Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc García-Fuentes, Eduardo author Santiago-Fernández, Concepción author Gutiérrez-Repiso, Carolina author Mayas, María D author Oliva-Olivera, Wilfredo author Coín-Aragüez, Leticia author Alcaide, Juan author Ocaña-Wilhelmi, Luis author Vendrell, Joan author Tinahones, Francisco J author Garrido-Sánchez, Lourdes author 2015-11-30 BACKGROUND: A key role for HIF-1α in the promotion and maintenance of dietary obesity has been proposed. We analyzed the association between hypoxia and de novo lipogenesis in human adipose tissue. METHODS: We studied HIF-1α mRNA and protein expression in fasting status in visceral adipose tissue (VAT) from non-obese and morbidly obese subjects, and in VAT from wild-type and ob/ob C57BL6J mice in both fasting and feeding status. We also analyzed the effect of hypoxia on the VAT mRNA expression of genes involved in lipogenesis. RESULTS: HIF-1α was increased in VAT from morbidly obese subjects. In fasting status, C57BL6J ob/ob mice had a higher VAT HIF-1α mRNA expression than C57BL6J wild-type mice. In feeding status, VAT HIF-1α mRNA expression significantly increased in C57BL6J wild-type, but not in C57BL6J ob/ob mice. In humans, HIF-1α mRNA expression correlated positively with body mass index and insulin resistance. VAT HIF-1α mRNA expression correlated negatively with ACC1, PDHB and SIRT3 mRNA expression, and positively with PPAR-γ. VAT explants incubated in hypoxia showed reduced SIRT3 and increased PPAR-γ, SREBP-1c, ACLY, ACC1 and FASN mRNA expression. CONCLUSIONS: Morbidly obese subjects have a higher level of VAT HIF-1α. Postprandial status is associated with an increase in HIF-1α mRNA expression in C57BL6J wild-type mice. Hypoxia alters the mRNA expression of genes involved in de novo lipogenesis in human VAT. 10.1186/s12967-015-0732-5 1479-5876 Journal of Translational Medicine http://hdl.handle.net/10668/2449 26619907 http://hdl.handle.net/20.500.12105/17106 Adipogénesis Tejido adiposo Índice de masa corporal Dieta Ayuno Factor 1 inducible por hipoxia Resistencia a la insulina Grasa intraabdominal Lipogénesis Ratones Humanos Obesidad mórbida PPAR gamma ARN mensajero Proteína 1 de unión a elementos reguladores de esteroles Hypoxia is associated with a lower expression of genes involved in lipogenesis in visceral adipose tissue.