2026-06-14T03:32:04Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/171052024-11-28T14:58:11Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927col_20.500.12105_16961
Repisalud
author
Martínez-Huélamo, Miriam
author
Tulipani, Sara
author
Jáuregui, Olga
author
Valderas-Martínez, Palmira
author
Vallverdú-Queralt, Anna
author
Estruch, Ramón
author
Torrado, Xavier
author
Lamuela-Raventós, Rosa M
authoraffiliation
[Martínez-Huélamo,M; Torrado,X; Lamuela-Raventós,RM] University of Barcelona, Department of Nutrition & Food Science XARTA INSA, School of Pharmacy, Barcelona, Spain. [Martínez-Huélamo,M; Valderas-Martínez,P; Vallverdú-Queralt,A; Estruch,R; Lamuela-Raventós,RM] Carlos III Health Institute, Cemter of Biomedical Network Research Pathophysiology Obesity & Nutrition CIBEROBN, Madrid, Spain. [Tulipani,S] Biomedical Research Institute (IBIMA). Service of Endocrinology and Nutrition, Hospital Virgen de la Victoria. University of Malaga, Malaga, Spain. [Jaúregui,O] Universidad de Barcelona CCiTUB, Center Scientific & Technological, Barcelona, Spain. [Valderas-Martínez,P; Estruch,R] University of Barcelona, Department of Internal Medicine, Hospital Clinic, Institute of Biomedical Investigation August Pi I Sunyer IDIBAPS, Barcelona, Spain. [Vallverdú-Queralt,A] INRA, UMR1083 Sciences for Oenology, Montpellier, France.
2024-01-15T18:17:52Z
2024-01-15T18:17:52Z
2015-11-16
10.3390/molecules201119702
1420-3049
Molecules (Basel, Switzerland)
http://hdl.handle.net/10668/2361
26580589
http://hdl.handle.net/20.500.12105/17105
An UHPLC-MS/MS method for the quantification of tomato phenolic metabolites in human fluids was optimized and validated, and then applied in a pilot dietary intervention study with healthy volunteers. A 5-fold gain in speed (3.5 min of total run); 7-fold increase in MS sensitivity and 2-fold greater efficiency (50% peak width reduction) were observed when comparing the proposed method with the reference-quality HPLC-MS/MS system, whose assay performance has been previously documented. The UHPLC-MS/MS method led to an overall improvement in the limits of detection (LOD) and quantification (LOQ) for all the phenolic compounds studied. The recoveries ranged between 68% and 100% in urine and 61% and 100% in plasma. The accuracy; intra- and interday precision; and stability met with the acceptance criteria of the AOAC International norms. Due to the improvements in the analytical method; the total phenolic metabolites detected in plasma and urine in the pilot intervention study were 3 times higher than those detected by HPLC-MS/MS. Comparing with traditional methods; which require longer time of analysis; the methodology described is suitable for the analysis of phenolic compounds in a large number of plasma and urine samples in a reduced time frame.
eng
Polyphenol metabolites
Validation
Tomato
Sauce
Microbiota
Metabolites
Biosamples
Blood
Urine
Fluidos corporales
Cromatografía líquida de alta presión
Voluntarios sanos
Límite de detección
Fenoles
Humanos
Espectrometría de masas en tándem
Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples.
research article