2026-06-14T03:37:39Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/171052024-11-28T14:58:11Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927col_20.500.12105_16961

00925njm 22002777a 4500 dc Martínez-Huélamo, Miriam author Tulipani, Sara author Jáuregui, Olga author Valderas-Martínez, Palmira author Vallverdú-Queralt, Anna author Estruch, Ramón author Torrado, Xavier author Lamuela-Raventós, Rosa M author 2015-11-16 An UHPLC-MS/MS method for the quantification of tomato phenolic metabolites in human fluids was optimized and validated, and then applied in a pilot dietary intervention study with healthy volunteers. A 5-fold gain in speed (3.5 min of total run); 7-fold increase in MS sensitivity and 2-fold greater efficiency (50% peak width reduction) were observed when comparing the proposed method with the reference-quality HPLC-MS/MS system, whose assay performance has been previously documented. The UHPLC-MS/MS method led to an overall improvement in the limits of detection (LOD) and quantification (LOQ) for all the phenolic compounds studied. The recoveries ranged between 68% and 100% in urine and 61% and 100% in plasma. The accuracy; intra- and interday precision; and stability met with the acceptance criteria of the AOAC International norms. Due to the improvements in the analytical method; the total phenolic metabolites detected in plasma and urine in the pilot intervention study were 3 times higher than those detected by HPLC-MS/MS. Comparing with traditional methods; which require longer time of analysis; the methodology described is suitable for the analysis of phenolic compounds in a large number of plasma and urine samples in a reduced time frame. 10.3390/molecules201119702 1420-3049 Molecules (Basel, Switzerland) http://hdl.handle.net/10668/2361 26580589 http://hdl.handle.net/20.500.12105/17105 Polyphenol metabolites Validation Tomato Sauce Microbiota Metabolites Biosamples Blood Urine Fluidos corporales Cromatografía líquida de alta presión Voluntarios sanos Límite de detección Fenoles Humanos Espectrometría de masas en tándem Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples.