2026-06-14T06:44:35Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/171032024-11-28T15:51:27Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Blanco, Eduardo
author
Galeano, Pablo
author
Holubiec, Mariana I
author
Romero, Juan I
author
Logica, Tamara
author
Rivera, Patricia
author
Pavón, Francisco-Javier
author
Suarez, Juan
author
Capani, Francisco
author
Rodríguez de Fonseca, Fernando
authoraffiliation
[Blanco,E,; Rivera,P; Pavón,FJ; Suarez.K: Rodríguez de Fonseca,F] Unidad de Gestión Clínica de Salud Mental, Laboratorio de Medicina Regenerativa, Instituto de Investigación Biomédica de Málaga (IBIMA). Hospital Universitario Regional de Málaga. Universidad de Málaga, Málaga, Spain. [Blanco,E] Departament de Pedagogia i Psicologia, Facultat d’Educació, Psicologia i Treball Social, Universitat de Lleida, Lleida, Spain. [Galeano,P] Instituto de Investigaciones Bioquímicas de Buenos Aires – Consejo Nacional de Investigaciones Científicas y Técnicas. Fundación Instituto Leloir, Buenos Aires, Argentina. [Galeano,P; Holubiec,MI; Romero,JI; Logica,T; Campani,] Facultad de Medicina, Instituto de Investigaciones Cardiológicas “Prof. Dr. Alberto C. Taquini”, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Buenos Aires, Argentina.
2024-01-15T18:17:50Z
2024-01-15T18:17:50Z
2015-11-03
10.3389/fnana.2015.00141
1662-5129
Frontiers in Neuroanatomy
http://hdl.handle.net/10668/2338
26578900
http://hdl.handle.net/20.500.12105/17103
Perinatal asphyxia (PA) is an obstetric complication that strongly affects the CNS. The endocannabinoid system (ECS) is a lipid transmitter system involved in several physiological processes including synaptic plasticity, neurogenesis, memory, and mood. Endocannabinoids, and other acylethanolamides (AEs) without endocannabinoid activity, have recently received growing attention due to their potential neuroprotective functions in neurological disorders, including cerebral ischemia. In the present study, we aimed to analyze the changes produced by PA in the major metabolic enzymes and receptors of the ECS/AEs in the hippocampus using a rodent model of PA. To induce PA, we removed uterine horns from ready-to-deliver rats and immersed them into a water bath during 19 min. Animals delivered spontaneously or by cesarean section were employed as controls. At 1 month of age, cognitive functions were assessed and immunohistochemical procedures were carried out to determine the expression of NeuN and glial fibrillary acidic protein, enzymes responsible for synthesis (DAGLα and NAPE-PLD) and degradation (FAAH) of ECS/AEs and their receptors (CB1 and PPARα) in the hippocampus. Postweaned asphyctic rats showed impaired recognition and spatial reference memory that were accompanied by hippocampal astrogliosis and changes in the expression of enzymes and receptors. The most remarkable findings in asphyctic rats were a decrease in the expression of NAPE-PLD and PPARα in both hippocampal areas CA1 and CA3. In addition, postweaned cesarean delivery rats showed an increase in the immunolabeling for FAAH in the hippocampal CA3 area. Since, NAPE-PLD and PPARα are proteins that participate in the biochemical process of AEs, specially the neuroprotective oleoylethanolamide, these results suggest that PA dysregulates this system. These data encourage conducting future studies using AEs as potential neuroprotective compounds in animal models of PA.
eng
Perinatal asphyxia
Hippocampus
Memory
DAGLα
NAPE-PLD
CB1
PPARα
FAAH
Animales
Asfixia
Atención
Baños
Procesos bioquímicos
Isquemia cerebral
Cesárea
Cognición
Endocannabinoides
Proteína ácida fibrilar de la glía
Memoria
Modelos animales
Enfermedades del sistema nervioso
Neurogénesis
Plasticidad neural
Ácidos oleicos
Embarazo
Procesos fisiológicos
Ratas
Perinatal asphyxia results in altered expression of the hippocampal acylethanolamide/endocannabinoid signaling system associated to memory impairments in postweaned rats.
research article