2026-04-29T12:24:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/170782024-11-28T14:45:57Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Mediavilla-Gradolph, María Concepción
author
De Toro-Peinado, Inmaculada
author
Bermúdez-Ruiz, María Pilar
author
García-Martínez, María de los Ángeles
author
Ortega-Torres, María
author
Montiel Quezel-Guerraz, Natalia
author
Palop-Borrás, Begoña
authoraffiliation
[Mediavilla-Gradolph,MC; De Toro-Peinado,I; Bermúdez-Ruiz,MP; García-Martínez,MA; Ortega-Torres,M; Palop-Borrás,B] Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, Malaga, Spain. [Montiel Quezel-Guerraz,N] Microbiology Unit, Hospital Costa del Sol, Marbella, Spain.
2024-01-15T18:17:24Z
2024-01-15T18:17:24Z
2015
10.1155/2015/854078
2314-6141
BioMed Research International
http://hdl.handle.net/10668/2240
26106617
http://hdl.handle.net/20.500.12105/17078
The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species.
eng
Humanos
Genotipo
Infecciones por micobacterias no tuberculosas
Micobacterias no tuberculosas
Espectrometría de masas con desorción-ionización láser asistida por matriz
Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens
research article