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oai:repisalud.isciii.es:20.500.12105/170782024-11-28T14:45:57Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Mediavilla-Gradolph, María Concepción author De Toro-Peinado, Inmaculada author Bermúdez-Ruiz, María Pilar author García-Martínez, María de los Ángeles author Ortega-Torres, María author Montiel Quezel-Guerraz, Natalia author Palop-Borrás, Begoña author 2015 The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species. 10.1155/2015/854078 2314-6141 BioMed Research International http://hdl.handle.net/10668/2240 26106617 http://hdl.handle.net/20.500.12105/17078 Humanos Genotipo Infecciones por micobacterias no tuberculosas Micobacterias no tuberculosas Espectrometría de masas con desorción-ionización láser asistida por matriz Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens