2026-06-14T03:45:12Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/170672024-11-28T15:29:24Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Rueda-Martínez, Carmen author Lamas, Óscar author Mataró, María José author Robledo-Carmona, Juan author Sánchez-Espín, Gemma author Jiménez-Navarro, Manuel author Such-Martínez, Miguel author Fernández, Borja author 2014-05-19 Dilatation of the ascending aorta (AAD) is a prevalent aortopathy that occurs frequently associated with bicuspid aortic valve (BAV), the most common human congenital cardiac malformation. The molecular mechanisms leading to AAD associated with BAV are still poorly understood. The search for differentially expressed genes in diseased tissue by quantitative real-time PCR (qPCR) is an invaluable tool to fill this gap. However, studies dedicated to identify reference genes necessary for normalization of mRNA expression in aortic tissue are scarce. In this report, we evaluate the qPCR expression of six candidate reference genes in tissue from the ascending aorta of 52 patients with a variety of clinical and demographic characteristics, normal and dilated aortas, and different morphologies of the aortic valve (normal aorta and normal valve n = 30; dilated aorta and normal valve n = 10; normal aorta and BAV n = 4; dilated aorta and BAV n = 8). The expression stability of the candidate reference genes was determined with three statistical algorithms, GeNorm, NormFinder and Bestkeeper. The expression analyses showed that the most stable genes for the three algorithms employed were CDKN1β, POLR2A and CASC3, independently of the structure of the aorta and the valve morphology. In conclusion, we propose the use of these three genes as reference genes for mRNA expression analysis in human ascending aorta. However, we suggest searching for specific reference genes when conducting qPCR experiments with new cohort of samples. 10.1371/journal.pone.0097449 1932-6203 PloS One http://hdl.handle.net/10668/1639 24841551 http://hdl.handle.net/20.500.12105/17067 Enfermedades de las Válvulas Cardíacas Válvula Aórtica Algoritmos Aneurisma de la Aorta Reacción en Cadena en Tiempo Real de la Polimerasa Demografía Expresión Génica Dilatación Patológica Reacción en Cadena de la Polimerasa ADN Complementario Desnaturalización de Ácido Nucleico Humanos Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta.