2026-04-27T03:43:11Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/170512024-11-28T15:30:03Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Garrido-Sánchez, Lourdes
author
Escote, Xavier
author
Coín-Aragüez, Leticia
author
Fernández-García, José Carlos
author
El Bekay, Rajaa
author
Vendrell, Joan
author
García-Fuentes, Eduardo
author
Tinahones, Francisco J
authoraffiliation
[Garrido-Sánchez,L; Escote,X; Vendrell,J] Endocrinology and Diabetes Unit, Joan XXIII University Hospital, IISPV, Universitat Rovira i Virgili, Tarragona, Spain. [Garrido-Sánchez,L; Escote,X; Vendrell,J] CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Tarragona, Spain. [Coín-Aragüez,L; Fernández-García,JC; El Bekay,R; Tinahones,FJ] Servicio de Endocrinología y Nutrición, Hospital Universitario Virgen de la Victoria, Málaga, Spain. [Coín-Aragüez,L; El Bekay,R; García-Fuentes,E; Tinahones,FJ] Ciber Fisiopatología Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Málaga, Spain. [El Bekay,R; García-Fuentes,E] Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Málaga, Spain. [García-Fuentes,E] Servicio de Endocrinología y Nutrición, Hospital Regional Universitario Carlos Haya, Málaga, Spain.
2024-01-15T18:17:00Z
2024-01-15T18:17:00Z
2013-05-20
http://hdl.handle.net/10668/1156
http://hdl.handle.net/20.500.12105/17051
23700440
10.1371/journal.pone.0063937
1932-6203
PloS one
OBJECTIVE: Munc18c is associated with glucose metabolism and could play a relevant role in obesity. However, little is known about the regulation of Munc18c expression. We analyzed Munc18c gene expression in human visceral (VAT) and subcutaneous (SAT) adipose tissue and its relationship with obesity and insulin. MATERIALS AND METHODS: We evaluated 70 subjects distributed in 12 non-obese lean subjects, 23 overweight subjects, 12 obese subjects and 23 nondiabetic morbidly obese patients (11 with low insulin resistance and 12 with high insulin resistance). RESULTS: The lean, overweight and obese persons had a greater Munc18c gene expression in adipose tissue than the morbidly obese patients (p<0.001). VAT Munc18c gene expression was predicted by the body mass index (B = -0.001, p = 0.009). In SAT, no associations were found by different multiple regression analysis models. SAT Munc18c gene expression was the main determinant of the improvement in the HOMA-IR index 15 days after bariatric surgery (B = -2148.4, p = 0.038). SAT explant cultures showed that insulin produced a significant down-regulation of Munc18c gene expression (p = 0.048). This decrease was also obtained when explants were incubated with liver X receptor alpha (LXRα) agonist, either without (p = 0.038) or with insulin (p = 0.050). However, Munc18c gene expression was not affected when explants were incubated with insulin plus a sterol regulatory element-binding protein-1c (SREBP-1c) inhibitor (p = 0.504). CONCLUSIONS: Munc18c gene expression in human adipose tissue is down-regulated in morbid obesity. Insulin may have an effect on the Munc18c expression, probably through LXRα and SREBP-1c.
eng
Resistencia a la Insulina
Obesidad Mórbida
Tejido Adiposo
Glucosa
HDL-Colesterol
Regulación de la Expresión Génica
Proteínas Munc18
Munc18c in adipose tissue is downregulated in obesity and is associated with insulin.
research article