2026-07-21T16:42:27Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/170482024-11-28T15:00:17Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Alen, Francisco
author
Crespo, Inmaculada
author
Ramírez-López, María Teresa
author
Jagerovic, Nadine
author
Goya, Pilar
author
Rodríguez de Fonseca, Fernando
author
Gómez de Heras, Raquel
author
Orio, Laura
authoraffiliation
[Alen,F; Crespo,I Ramírez-López,MT; Rodríguez de Fonseca,F; Gómez de Heras,R; Orio,L] Departamento de Psicobiología, Facultad de Psicología, Universidad Complutense de Madrid, Spain. [Jagerovic,N; Goya,P] Instituto de Química Médica, Centro Superior de Investigaciones Científicas, Madrid, Spain. [Rodríguez de Fonseca,F] Hospital Carlos Haya, Fundación Pública Andaluza para la Investigación en Málaga en Biomedicina y Salud (FIMABIS), Málaga, Spain. Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain.
2024-01-15T18:16:58Z
2024-01-15T18:16:58Z
2013-04-02
10.1371/journal.pone.0060918
1932-6203
PloS one
http://hdl.handle.net/10668/1419
23565287
http://hdl.handle.net/20.500.12105/17048
Ghrelin is an endogenous regulator of energy homeostasis synthesized by the stomach to stimulate appetite and positive energy balance. Similarly, the endocannabinoid system is part of our internal machinery controlling food intake and energy expenditure. Both peripheral and central mechanisms regulate CB1-mediated control of food intake and a functional relationship between hypothalamic ghrelin and cannabinoid CB1 receptor has been proposed. First of all, we investigated brain ghrelin actions on food intake in rats with different metabolic status (negative or equilibrate energy balance). Secondly, we tested a sub-anxiogenic ultra-low dose of the CB1 antagonist SR141716A (Rimonabant) and the peripheral-acting CB1 antagonist LH-21 on ghrelin orexigenic actions. We found that: 1) central administration of ghrelin promotes food intake in free feeding animals but not in 24 h food-deprived or chronically food-restricted animals; 2) an ultra-low dose of SR141716A (a subthreshold dose 75 folds lower than the EC50 for induction of anxiety) completely counteracts the orexigenic actions of central ghrelin in free feeding animals; 3) the peripheral-restricted CB1 antagonist LH-21 blocks ghrelin-induced hyperphagia in free feeding animals. Our study highlights the importance of the animaĺs metabolic status for the effectiveness of ghrelin in promoting feeding, and suggests that the peripheral endocannabinoid system may interact with ghrelińs signal in the control of food intake under equilibrate energy balance conditions.
eng
Animales
Ghrelina
Masculino
Piperidinas
Ratas
Ratas Wistar
Ingestión de Alimentos
Receptor cannabinoide CB1
Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.
research article