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oai:repisalud.isciii.es:20.500.12105/170462024-11-28T15:38:41Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author Henn, Dominic author Bandner-Risch, Doris author Perttunen, Hilja author Schmied, Wolfram author Porras, Carlos author Ceballos, Francisco author RodrÍguez-Losada, Noela author Schäfers, Hans-Joachim authoraffiliation [Henn,D; Bandner-Risch,D; Perttunen,H; Schmied,W; Schäfers,H-J] Department of Thoracic and Cardiovascular Surgery, Saarland University Hospital, Homburg/Saar, Germany. [Porras,C] Department of Cardiac Surgery, Hospital Universitario Virgen de la Victoria de Málaga, Spain. [Ceballos,F] Department of Genetics, Faculty of Biology, University of Santiago de Compostela, Spain. [Rodríguez-Losada,N] Foundation IMABIS, Carlos Haya University Hospital, Avenida Carlos Haya, Malaga, Spain. 2024-01-15T18:16:57Z 2024-01-15T18:16:57Z 2013-01-11 10.1371/journal.pone.0054132 1932-6203 PloS One http://hdl.handle.net/10668/877 23326585 http://hdl.handle.net/20.500.12105/17046 Hypertension and congenital aortic valve malformations are frequent causes of ascending aortic aneurysms. The molecular mechanisms of aneurysm formation under these circumstances are not well understood. Reference genes for gene activity studies in aortic tissue that are not influenced by aortic valve morphology and its hemodynamic consequences, aortic dilatation, hypertension, or antihypertensive medication are not available so far. This study determines genes in ascending aortic tissue that are independent of these parameters. Tissue specimens from dilated and undilated ascending aortas were obtained from 60 patients (age ≤70 years) with different morphologies of the aortic valve (tricuspid undilated n = 24, dilated n = 11; bicuspid undilated n = 6, dilated n = 15; unicuspid dilated n = 4). Of the studied individuals, 36 had hypertension, and 31 received ACE inhibitors or AT1 receptor antagonists. The specimens were obtained intraoperatively from the wall of the ascending aorta. We analyzed the expression levels of 32 candidate reference genes by quantitative RT-PCR (RT-qPCR). Differential expression levels were assessed by parametric statistics. The expression analysis of these 32 genes by RT-qPCR showed that EIF2B1, ELF1, and PPIA remained constant in their expression levels in the different specimen groups, thus being insensitive to aortic valve morphology, aortic dilatation, hypertension, and medication with ACE inhibitors or AT1 receptor antagonists. Unlike many other commonly used reference genes, the genes EIF2B1, ELF1, and PPIA are neither confounded by aortic comorbidities nor by antihypertensive medication and therefore are most suitable for gene expression analysis of ascending aortic tissue. eng Aneurisma de la Aorta Enfermedades de las Válvulas Cardíacas Hipertensión Adulto Estudios de Asociación Genética Expresión Génica Tejidos Humanos Factor 2B Eucariótico de Iniciación Reacción en Cadena de la Polimerasa de Transcriptasa Inversa Femenino Masculino Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms. research article