2026-08-29T14:55:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/170412024-11-28T14:44:42Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927col_20.500.12105_16961col_20.500.12105_16980
Repisalud
author
Palacín, Arantxa
author
Rivas, Luis A.
author
Gómez-Casado, Cristina
author
Aguirre, Jacobo
author
Tordesillas, Leticia
author
Bartra, Joan
author
Carrillo, Teresa
author
Cuesta-Herranz, Javier
author
Cumplido Bonny, José A.
author
Flores, Enrique
author
García-Alvarez-Eire, Mar G.
author
García-Nuñez, Ignacio
author
Fernández, Francisco J.
author
Gamboa, Pedro
author
Muñoz, Rosa
author
Sánchez-Monge, Rosa
author
Torres, Maria
author
Varela Losada, Susana
author
Villalba, Mayte
author
Parro, Victor
author
Blanca, Miguel
author
Salcedo, Gabriel
author
Díaz-Perales, Araceli
authoraffiliation
[Palacín,A; Gómez-Casado,C; Tordesillas,L; Sánchez-Monge,R; Díaz-Perales,A] Centro de Biotecnología y Genómica de Plantas, Campus de Montegancedo, Pozuelo de Alarcón, Madrid, Spain. [Rivas,LA; Aguirre,J; Parro,V] Departamento de Evolución Molecular, Centro de Astrobiología (INTA-CSIC), Torrejón de Ardoz, Madrid, Spain. [Bartra,J; Muñoz,R] Unitat d’Allèrgia, Servei Pneumologia i Allèrgia Respiratòria, Hospital Clínic, Universitat de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (CIBERES), Barcelona, Spain. [Blanco,C; Vega,F] Servicio de Alergia, Hospital Universitario de la Princesa, Instituto de Investigación Sanitaria Princesa (IP), Madrid, Spain. [Carrillo,T; Cumplido Bonny,JA] Servicio de Alergología, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, Spain. [Cuesta-Herranz,J] Servicio de Alergia, Fundación Jiménez Díaz, Madrid, Spain. [Flores,E; Fernández,FJ] Unidad de Alergia, Hospital General Universitario, Alicante, Spain. [García-Alvarez-Eire,MG; Varela Losada,S] Unidad de Alergología, Complexo Hospitalario, Ourense, Spain. [García-Nuñez,I; Blanca,M] Laboratorio de Investigación, Fundación IMABIS-Carlos Haya Hospital, Hospital Civil, Málaga, Spain. [Gamboa,P] Servicio de Alergia, Hospital de Basurto, Bilbao, Spain. [Torres,M; Villalba,M] Departamento de Bioquímica y Biología Molecular I, Facultad de Químicas, Universidad Complutense, Madrid, Spain. [Salcedo,G]Departamento de Biotecnología, ETSI Agrónomos, Universidad Politécnica, Madrid, Spain.
2024-01-15T18:16:51Z
2024-01-15T18:16:51Z
2012-09-07
10.1371/journal.pone.0044088
1932-6203
PloS one
http://hdl.handle.net/10668/1409
22970164
http://hdl.handle.net/20.500.12105/17041
Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited >50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy.
eng
España
Geografía
Reacciones cruzadas
Hipersensibilidad a los alimentos
Inmunización
Inmunoensayo
Inmunoglobulina E
Peso molecular
Proteínas de plantas
Polen
Análisis por matrices de proteínas
The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray
research article