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oai:repisalud.isciii.es:20.500.12105/170302024-11-28T15:05:27Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author Clemente-Postigo, Mercedes author Queipo-Ortuño, María Isabel author Fernández-García, Diego José author Gómez-Huelgas, Ricardo author Tinahones Madueño, Francisco José author Cardona Díaz, Fernando authoraffiliation [Clemente-Postigo,M; Queipo-Ortuño,MI; Fernández-García,DJ] Laboratorio de Investigaciones Biomédicas del Hospital Universitario Virgen de la Victoria, Málaga (Fundación IMABIS), Spain. [Queipo-Ortuño,MI; Fernández-García,DJ; Gomez-Huelgas,R; Tinahones FJ; Cardona,F] CIBER Fisiopatología de la Obesidad y la Nutrición, Spain. [Fernández-García,DJ; Tinahones Madueño,FJ] Servicio Endocrinología y Nutrición del Hospital Universitario Virgen de la Victoria, Málaga, Spain. [Gómez-Huelgas,R] Servicio de Medicina Interna del Hospital Regional Carlos Haya, Spain. 2024-01-15T18:16:39Z 2024-01-15T18:16:39Z 2011-09-22 10.1371/journal.pone.0024783 1932-6203 PloS one http://hdl.handle.net/10668/694 21966368 http://hdl.handle.net/20.500.12105/17030 BACKGROUND: Adipose tissue lipid storage and processing capacity can be a key factor for obesity-related metabolic disorders such as insulin resistance and diabetes. Lipid uptake is the first step to adipose tissue lipid storage. The aim of this study was to analyze the gene expression of factors involved in lipid uptake and processing in subcutaneous (SAT) and visceral (VAT) adipose tissue according to body mass index (BMI) and the degree of insulin resistance (IR). METHODS AND PRINCIPAL FINDINGS: VLDL receptor (VLDLR), lipoprotein lipase (LPL), acylation stimulating protein (ASP), LDL receptor-related protein 1 (LRP1) and fatty acid binding protein 4 (FABP4) gene expression was measured in VAT and SAT from 28 morbidly obese patients with Type 2 Diabetes Mellitus (T2DM) or high IR, 10 morbidly obese patients with low IR, 10 obese patients with low IR and 12 lean healthy controls. LPL, FABP4, LRP1 and ASP expression in VAT was higher in lean controls. In SAT, LPL and FABP4 expression were also higher in lean controls. BMI, plasma insulin levels and HOMA-IR correlated negatively with LPL expression in both VAT and SAT as well as with FABP4 expression in VAT. FABP4 gene expression in SAT correlated inversely with BMI and HOMA-IR. However, multiple regression analysis showed that BMI was the main variable contributing to LPL and FABP4 gene expression in both VAT and SAT. CONCLUSIONS: Morbidly obese patients have a lower gene expression of factors related with lipid uptake and processing in comparison with healthy lean persons. eng LRP1 protein, human acylation stimulating protein, human Proteínas de Unión a Ácidos Grasos Índice de masa corporal Diabetes Mellitus Tipo 2 FABP4 protein, human Expresión genética Resistencia a la insulina Péptidos y Proteínas de Señalización Intercelular Grasa intraabdominal Metabolismo de los Lípidos Lipoproteína Lipasa Proteina 1 Relacionada con Receptor de Lipoproteína de Baja Densidad Obesidad mórbida Reacción en Cadena de la Polimerasa de Transcriptasa Inversa Grasa subcutánea Adipose tissue gene expression of factors related to lipid processing in obesity. research article