2026-06-14T03:32:04Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/169292025-05-13T09:03:56Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Deni, Alessandro author Mistral De Pascali, Alessandra author Ortalli, Margherita author Balducelli, Emma author Provenzano, Michele author Ferrara, Francesca author Busutti, Marco author La Manna, Gaetano author Zammarchi, Lorenzo author Bartoloni, Alessandro author Caroti, Leonardo author Ibarra-Meneses, Ana Victoria author Carrillo, Eugenia author Comai, Giorgia author Varani, Stefania author 2024-01 Objectives: In immunocompromised patients, asymptomatic Leishmania infection can reactivate, and evolve to severe disease. To date, no test is considered the gold standard for the identification of asymptomatic Leishmania infection. A combination of methods was employed to screen for Leishmania infection in patients undergoing kidney transplant (KT). Methods: We employed polymerase chain reaction for the detection of parasitic DNA in peripheral blood, Western blot to identify serum immunoglobulin G and whole blood assay to detect cytokines/chemokines after stimulation of whole blood with parasitic antigen. Results: One-hundred twenty patients residing in Italy were included in the study at the time of KT. Each patient that tested positive to at least one test was considered as Leishmania positive. Fifty out of 120 patients (42%) tested positive for one or more tests. The detection of specific cell-mediated response (32/111, 29%) was the most common marker of Leishmania infection, followed by a positive serology (24/120, 20%). Four patients (3%) harbored parasitic DNA in the blood. Conclusion: Our findings underline the high prevalence of asymptomatic Leishmania infection in patients undergoing KT in Italy, who are potentially at-risk for parasite reactivation and can benefit from an increased vigilance. Understanding the clinical relevance of these findings deserves further studies. Int J Infect Dis. 2024 Jan:138:81-83. 10.1016/j.ijid.2023.11.012 1878-3511 International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 37995832 http://hdl.handle.net/20.500.12105/16929 Asymptomatic Leishmania infection Kidney transplant recipients Screening tests Identification of asymptomatic Leishmania infection in patients undergoing kidney transplant using multiple tests