2026-08-05T09:54:33Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/168692025-05-05T09:49:04Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Sebuyoya, Ravery
author
Valverde, Alejandro
author
Moranova, Ludmila
author
Strmiskova, Johana
author
Hrstka, Roman
author
Ruiz-Valdepeñas Montiel, Víctor
author
Pingarrón, José M
author
Barderas Manchado, Rodrigo
author
Campuzano, Susana
author
Bartosik, Martin
funder
Czech Health Research Council
funder
National Institute for Cancer Research (República Checa)
funder
Unión Europea. Comisión Europea. NextGenerationEU
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid (España)
funder
Agencia Estatal de Investigación (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Complutense University of Madrid (España)
2023-12-21T20:59:32Z
2023-12-21T20:59:32Z
2023
Sensors & Actuators: B. Chemical.2023; 394:134375.
10.1016/j.snb.2023.134375
0925-4005
Sensors and Actuators B: Chemical
http://hdl.handle.net/20.500.12105/16869
DNA point mutation in a BRAF proto-oncogene, V600E, is considered an important prognostic and predictive biomarker in various types of cancer, such as melanoma or colorectal cancer. We report here a novel electrochemical (EC) bioplatform for the analysis of BRAF V600E mutation coupled with rolling circle amplification (RCA) and locked nucleic acid (LNA) capture probes. A dual detection system was implemented, whereby two padlock probes complementary to either wild-type (wt) BRAF gene or DNA with V600E mutation (mut) led to amplification of wt or mut variant, respectively. Hybridization with specific LNA capture probes then increased the assay specificity, while EC detection provided rapid measurement times. The bioplatform was applied to analyze BRAF V600E mutation of cancer cells and tumor tissues from patients with melanoma or colorectal cancer. This is the first RCA-based EC bioplatform for BRAF analysis in a dual format without using PCR or sophisticated instrumentation.
eng
BRAF V600E mutation
Tumor biomarker
Electrochemical analysis
Rolling circle amplification
Dual detection
Locked nucleic acid
Dual detection system for cancer-associated point mutations assisted by a multiplexed LNA-based amperometric bioplatform coupled with rolling circle amplification
research article
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