2026-08-29T14:07:47Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/168482024-09-27T21:12:41Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Llorente, Francisco
author
Pérez-Ramírez, Elisa
author
Perez-Olmeda, Mayte
author
Dafouz-Bustos, Desirée
author
Fernández-Pinero, Jovita
author
Martínez-Cortés, Mercedes
author
Jiménez-Clavero, Miguel Ángel
funder
Comunidad de Madrid (España)
2023-12-19T20:58:33Z
2023-12-19T20:58:33Z
2023-11-16
Pathogens. 2023 Nov 16;12(11):1360.
10.3390/pathogens12111360
2076-0817
Pathogens (Basel, Switzerland)
38003824
http://hdl.handle.net/20.500.12105/16848
The presence of SARS-CoV-2 antibodies was examined over 7 months in a population of essential service workers exposed during the first epidemic wave in Madrid (Spain). Results obtained with different serological assays were compared. Firstly, serum samples obtained in April 2020 were analyzed using eleven SARS-CoV-2 antibody detection methods, including seven ELISAs, two CLIAs and two LFAs. While all of the ELISA tests and the Roche eCLIA method showed good performance, it was poorer for the Abbott CLIA and LFA tests. Sera from 115 workers with serologically positive results in April were collected 2 and 7 months after the first sampling and were analyzed using five of the tests previously assessed. The results showed that while some ELISA tests consistently detected the presence of anti-SARS-CoV-2 antibodies even 7 months after first detection, other methods, such as the Abbott CLIA test, showed an important reduction in sensitivity for these mature antibodies. The sensitivity increased after establishing new cut-off values, calculated taking into account both recent and old infections, suggesting that an adjustment of assay parameters may improve the detection of individuals exposed to the infection.
eng
CLIA
COVID-19
ELISA
LFA
SARS-CoV-2
Serologic assays
The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in Serosurveillance
research article
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URL
https://repisalud.isciii.es/bitstreams/b411ceae-fe08-4dab-8bac-6e60fb3cc482/download
File
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application/pdf
DetectionSARS-CoV-2Antibodies_2023.pdf
URL
https://repisalud.isciii.es/bitstreams/e287f293-c463-44c2-a029-cd6c14aa21d3/download
File
MD5
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text/plain
DetectionSARS-CoV-2Antibodies_2023.pdf.txt