2026-08-29T14:07:54Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/168482024-09-27T21:12:41Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Llorente, Francisco author Pérez-Ramírez, Elisa author Perez-Olmeda, Mayte author Dafouz-Bustos, Desirée author Fernández-Pinero, Jovita author Martínez-Cortés, Mercedes author Jiménez-Clavero, Miguel Ángel author 2023-11-16 The presence of SARS-CoV-2 antibodies was examined over 7 months in a population of essential service workers exposed during the first epidemic wave in Madrid (Spain). Results obtained with different serological assays were compared. Firstly, serum samples obtained in April 2020 were analyzed using eleven SARS-CoV-2 antibody detection methods, including seven ELISAs, two CLIAs and two LFAs. While all of the ELISA tests and the Roche eCLIA method showed good performance, it was poorer for the Abbott CLIA and LFA tests. Sera from 115 workers with serologically positive results in April were collected 2 and 7 months after the first sampling and were analyzed using five of the tests previously assessed. The results showed that while some ELISA tests consistently detected the presence of anti-SARS-CoV-2 antibodies even 7 months after first detection, other methods, such as the Abbott CLIA test, showed an important reduction in sensitivity for these mature antibodies. The sensitivity increased after establishing new cut-off values, calculated taking into account both recent and old infections, suggesting that an adjustment of assay parameters may improve the detection of individuals exposed to the infection. Pathogens. 2023 Nov 16;12(11):1360. 10.3390/pathogens12111360 2076-0817 Pathogens (Basel, Switzerland) 38003824 http://hdl.handle.net/20.500.12105/16848 CLIA COVID-19 ELISA LFA SARS-CoV-2 Serologic assays The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in Serosurveillance