2026-06-14T03:54:05Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/168472025-06-09T08:28:19Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Chaiyadet, Sujittra
author
Sotillo, Javier
author
Smout, Michael
author
Cooper, Martha
author
Doolan, Denise L.
author
Waardenberg, Ashley
author
Eichenberger, Ramon M
author
Field, Matt
author
Brindley, Paul J
author
Laha, Thewarach
author
Loukas, Alex
funder
National Health and Medical Research Council (Australia)
funder
NIH - National Cancer Institute (NCI) (Estados Unidos)
funder
Khon Kaen University (Tailandia)
funder
Ministerio de Ciencia e Innovación (España)
2023-12-19T19:41:42Z
2023-12-19T19:41:42Z
2023
bioRxiv. 2023 May 22:2023.05.22.540805.
10.1101/2023.05.22.540805
bioRxiv
37292777
http://hdl.handle.net/20.500.12105/16847
Chronic infection with O. viverrini has been linked to the development of cholangiocarcinoma (CCA), which is a major public health burden in the Lower Mekong River Basin countries, including Thailand, Lao PDR, Vietnam and Cambodia. Despite its importance, the exact mechanisms by which O. viverrini promotes CCA are largely unknown. In this study, we characterized different extracellular vesicle populations released by O. viverrini (OvEVs) using proteomic and transcriptomic analyses and investigated their potential role in host-parasite interactions. While 120k OvEVs promoted cell proliferation in H69 cells at different concentrations, 15k OvEVs did not produce any effect compared to controls. The proteomic analysis of both populations showed differences in their composition that could contribute to this differential effect. Furthermore, the miRNAs present in 120k EVs were analysed and their potential interactions with human host genes was explored by computational target prediction. Different pathways involved in inflammation, immune response and apoptosis were identified as potentially targeted by the miRNAs present in this population of EVs. This is the first study showing specific roles for different EV populations in the pathogenesis of a parasitic helminth, and more importantly, an important advance towards deciphering the mechanisms used in establishment of opisthorchiasis and liver fluke infection-associated malignancy.
eng
Opisthorchis viverrini
Extracellular vesicles
miRNAs
RNA-seq
50 cholangiocarcinoma
Small extracellular vesicles but not microvesicles from Opisthorchis viverrini promote cell proliferation in human cholangiocytes
preprint
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