2026-04-29T23:03:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/168212024-11-28T12:34:49Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_25179
Repisalud
author
Garcia-Barbazan, Irene
author
Torres-Cano, Alba
author
Garcia-Rodas, Rocio
author
Sachse, Martin
author
Luque, Daniel
author
Megías, Diego
author
Zaragoza, Oscar
funder
Agencia Estatal de Investigación (España)
funder
Ministerio de Ciencia e Innovación (España)
2023-12-15T18:51:32Z
2023-12-15T18:51:32Z
2024
mBio. 2024 Jan 16;15(1):e0254923.
10.1128/mbio.02549-23
2150-7511
mBio
38078728
http://hdl.handle.net/20.500.12105/16821
Cryptococcus neoformans is an excellent model to investigate fungal pathogenesis. This yeast can produce "titan cells," which are cells of an abnormally larger size that contribute to the persistence of the yeast in the host. In this work, we have used a new approach to characterize them by identifying drugs that inhibit this process. We have used a repurposing off-patent drug library, combined with an automatic method to image and analyze fungal cell size. In this way, we have identified many compounds that inhibit this transition. Interestingly, several compounds were antioxidants, allowing us to confirm that endogenous ROS and mitochondrial changes are important for titan cell formation. This work provides new evidence of the mechanisms required for titanization. Furthermore, the future characterization of the inhibitory mechanisms of the identified compounds by the scientific community will contribute to better understand the role of titan cells in virulence.
eng
Cryptococcus neoformans
Mitochondria
Off-patent drug repurposing
Reactive oxygen species
Titan-like cells
Accumulation of endogenous free radicals is required to induce titan-like cell formation in Cryptococcus neoformans
research article
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URL
https://repisalud.isciii.es/bitstreams/2ddbab09-18da-476a-ae5a-098668cf5042/download
File
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AccumulationEndogenousFreeRadicals_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/3fbe8b4d-6cf9-4cbe-9a02-c12405a1a39f/download
File
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AccumulationEndogenousFreeRadicals_2023.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/91293461-841f-418c-9d7b-93d2e4b14638/download
File
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AccumulationEndogenousFreeRadicals_2024.pdf.txt