2026-04-29T23:03:55Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/168212024-11-28T12:34:49Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_25179

00925njm 22002777a 4500 dc Garcia-Barbazan, Irene author Torres-Cano, Alba author Garcia-Rodas, Rocio author Sachse, Martin author Luque, Daniel author Megías, Diego author Zaragoza, Oscar author 2024 Cryptococcus neoformans is an excellent model to investigate fungal pathogenesis. This yeast can produce "titan cells," which are cells of an abnormally larger size that contribute to the persistence of the yeast in the host. In this work, we have used a new approach to characterize them by identifying drugs that inhibit this process. We have used a repurposing off-patent drug library, combined with an automatic method to image and analyze fungal cell size. In this way, we have identified many compounds that inhibit this transition. Interestingly, several compounds were antioxidants, allowing us to confirm that endogenous ROS and mitochondrial changes are important for titan cell formation. This work provides new evidence of the mechanisms required for titanization. Furthermore, the future characterization of the inhibitory mechanisms of the identified compounds by the scientific community will contribute to better understand the role of titan cells in virulence. mBio. 2024 Jan 16;15(1):e0254923. 10.1128/mbio.02549-23 2150-7511 mBio 38078728 http://hdl.handle.net/20.500.12105/16821 Cryptococcus neoformans Mitochondria Off-patent drug repurposing Reactive oxygen species Titan-like cells Accumulation of endogenous free radicals is required to induce titan-like cell formation in Cryptococcus neoformans