2026-06-14T03:45:07Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/167332025-04-01T09:58:55Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Rojas-Gómez, Amelia
author
Dosil, Sara G
author
Chichón, Francisco Javier
author
Fernández-Gallego, Nieves
author
Ferrarini, Alessia
author
Calvo, Enrique
author
Calzada-Fraile, Diego
author
Requena, Silvia
author
Otón, Joaquin
author
Serrano, Alvaro
author
Tarifa, Rocio
author
Arroyo, Montserrat
author
Sorrentino, Andrea
author
Pereiro, Eva
author
Vazquez, Jesus
author
Valpuesta, José M
author
Sánchez-Madrid, Francisco
author
Martín-Cófreces, Noa B
2023-11-27T15:06:13Z
2023-11-27T15:06:13Z
2023-06
J Extracell Vesicles. 2023 Jun;12(6):e12333.
10.1002/jev2.12333
2001-3078
Journal of extracellular vesicles
37328936
http://hdl.handle.net/20.500.12105/16733
Cell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modifications, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganelle contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.
eng
Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics.
journal article
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Chaperonin CCT controls_J Extracell Vesicles_2023.pdf
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text/plain
Chaperonin CCT controls_J Extracell Vesicles_2023.pdf.txt