2026-08-29T19:28:12Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/166942025-04-07T08:24:31Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19611

00925njm 22002777a 4500 dc Molina-de la Fuente, Irene author Sagrado Benito, María José author Ousley, Janet author Gisbert, Francisco de Bartolomé author Garcia, Luz author Gonzalez-Mora, Vicenta author Benito, Agustin author Chol, Buai Tut author Julla, Ahmed author Bakri, Abubakr author Nanclares, Carolina author Berzosa, Pedro author 2023 Artemisinin-combined treatments are the recommended first-line treatment of Plasmodium falciparum malaria, but they are being threatened by emerging artemisinin resistance. Mutations in pfk13 are the principal molecular marker for artemisinin resistance. This study characterizes the presence of mutations in pfk13 in P. falciparum in Western Equatoria State, South Sudan. We analyzed 468 samples from patients with symptomatic malaria and found 15 mutations (8 nonsynonymous and 7 synonymous). Each mutation appeared only once, and none were validated or candidate markers of artemisinin resistance. However, some mutations were in the same or following position of validated and candidate resistance markers, suggesting instability of the gene that could lead to resistance. The R561L nonsynonymous mutation was found in the same position as the R561H validated mutation. Moreover, the A578S mutation, which is widespread in Africa, was also reported in this study. We found a high diversity of other pfk13 mutations in low frequency. Therefore, routine molecular surveillance of resistance markers is highly recommended to promptly detect the emergence of resistance-related mutations and to limit their spread. Am J Trop Med Hyg. 2023 Sep 25;109(5):1072-1076. 10.4269/ajtmh.23-0382 1476-1645 The American journal of tropical medicine and hygiene 37748765 http://hdl.handle.net/20.500.12105/16694 Screening for K13-Propeller Mutations Associated with Artemisinin Resistance in Plasmodium falciparum in Yambio County (Western Equatoria State, South Sudan)