2026-05-20T22:56:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/166402024-09-27T08:03:13Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Orrantia, Ane
author
Vázquez-De Luis, Enrique
author
Astarloa-Pando, Gabirel
author
Terrén, Iñigo
author
Amarilla-Irusta, Ainhoa
author
Polanco-Alonso, Diego
author
González, Carmen
author
Uranga, Alasne
author
Carrascosa, Tomás
author
Mateos-Mazón, Juan J
author
García-Ruiz, Juan C
author
Callejas, Sergio
author
Quintas, Ana
author
Dopazo, Ana
author
Zenarruzabeitia, Olatz
author
Borrego, Francisco
funder
Asociación Española Contra el Cáncer
2023-11-06T11:29:27Z
2023-11-06T11:29:27Z
2022-10-21
iScience. 2022 Sep 28;25(10):105235.
10.1016/j.isci.2022.105235
2589-0042
iScience
36262311
http://hdl.handle.net/20.500.12105/16640
Autologous hematopoietic stem cell transplantation (autoHSCT) is a treatment option for hematological disorders and pediatric solid tumors. After an autoHSCT, natural killer (NK) cells are the first lymphocyte subset returning to normal levels. To uncover global changes during NK cell reconstitution after autoHSCT, we performed RNA-sequencing on NK cells before and after autoHSCT. Results showed profound changes in the gene expression profile of NK cells immediately after autoHSCT. Several biological processes including cell cycle, DNA replication and the mevalonate pathway were enriched. Significantly, we observed that following autoHSCT, NK cells acquired a decidual-like gene expression profile, including the expression of CD9. By using multiparametric flow cytometry, we confirmed the expansion of NK cells expressing CD9 immediately after autoHSCT, which exhibited higher granzyme B and perforin expression levels than CD9- NK cells. These results provide insights into the physiopathology of NK cells during their reconstitution after autoHSCT.
eng
In vivo expansion of a CD9+ decidual-like NK cell subset following autologous hematopoietic stem cell transplantation.
journal article
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URL
https://repisalud.isciii.es/bitstreams/cf8cd3ae-2428-4540-991e-264848d2929e/download
File
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application/pdf
In vivo expansion of a CD9_iScience_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/a975c1be-9a86-4042-a7c8-b70c886c775d/download
File
MD5
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text/plain
In vivo expansion of a CD9_iScience_2022.pdf.txt