2026-07-24T21:09:11Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/166402024-09-27T08:03:13Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Orrantia, Ane author Vázquez-De Luis, Enrique author Astarloa-Pando, Gabirel author Terrén, Iñigo author Amarilla-Irusta, Ainhoa author Polanco-Alonso, Diego author González, Carmen author Uranga, Alasne author Carrascosa, Tomás author Mateos-Mazón, Juan J author García-Ruiz, Juan C author Callejas, Sergio author Quintas, Ana author Dopazo, Ana author Zenarruzabeitia, Olatz author Borrego, Francisco author 2022-10-21 Autologous hematopoietic stem cell transplantation (autoHSCT) is a treatment option for hematological disorders and pediatric solid tumors. After an autoHSCT, natural killer (NK) cells are the first lymphocyte subset returning to normal levels. To uncover global changes during NK cell reconstitution after autoHSCT, we performed RNA-sequencing on NK cells before and after autoHSCT. Results showed profound changes in the gene expression profile of NK cells immediately after autoHSCT. Several biological processes including cell cycle, DNA replication and the mevalonate pathway were enriched. Significantly, we observed that following autoHSCT, NK cells acquired a decidual-like gene expression profile, including the expression of CD9. By using multiparametric flow cytometry, we confirmed the expansion of NK cells expressing CD9 immediately after autoHSCT, which exhibited higher granzyme B and perforin expression levels than CD9- NK cells. These results provide insights into the physiopathology of NK cells during their reconstitution after autoHSCT. iScience. 2022 Sep 28;25(10):105235. 10.1016/j.isci.2022.105235 2589-0042 iScience 36262311 http://hdl.handle.net/20.500.12105/16640 In vivo expansion of a CD9+ decidual-like NK cell subset following autologous hematopoietic stem cell transplantation.