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oai:repisalud.isciii.es:20.500.12105/166302024-09-21T13:50:45Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Cordoba, Raul author Alvarez, Beatriz author Masso, Pilar author Gonzalez, Fernando Ataulfo author Conejo, Luz author Velasco, Diego author Alonso, Juan-Manuel author Villarrubia, Jesus author Cava, Fernando author Llamas, Pilar author 2016-11 Double-hit lymphoma (DHL) is a rare type of lymphoma with concurrent chromosomal translocations of C-MYC with BCL2 or BCL6, associated with unfavorable prognosis. We describe a case of DHL in a 79-year-old female patient previously diagnosed with diffuse large B-cell lymphoma (DLBCL) with an early relapse in the ascitic fluid. A seven-color multiparametric flow cytometry immunophenotyping study of the ascitic fluid was carried out, and revealed 99.78% of large in size and high cellular complexity B-cells positive for CD19, CD10 (64.27%), CD45 dim, CD22 dim, CD25 (60%), CD43 bright, CD38 bright, and IgM (18.53%); and negative for CD20, CD5, CD23, CD79b, CD103, CD200, CD11c, and FMC7, and 78.99% without light chain expression and 21% with Lambda chain restriction. Due to the expression of CD19 and CD10 with overexpression of BCL-2 protein and due to CD43 and CD38 positivity detected, those cells showed features between DLBCL and Burkitt lymphoma. Fluorescence in situ hybridization (FISH) confirmed both c-MYC/IGH and BCL2/IGH rearrangement. Our findings may help to identify cases requiring additional cytogenetic analysis. © 2015 International Clinical Cytometry Society. Cytometry B Clin Cytom . 2016 Nov;90(6):543-545. 10.1002/cyto.b.21227 1552-4957 Cytometry. Part B, Clinical cytometry 25612555 http://hdl.handle.net/20.500.12105/16630 The utility of multiparametric seven-color flow cytometry in the detection of double hit lymphoma in ascitic fluid samples.