2026-04-18T16:00:26Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/166172024-09-27T09:58:26Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Bouthelier, Antonio
author
Fernández-Arroyo, Lucía
author
Mesa-Ciller, Claudia
author
Cibrian, Danay
author
Martín-Cófreces, Noa Beatriz
author
Castillo-González, Raquel
author
Calero, Macarena
author
Herráez-Aguilar, Diego
author
Guajardo-Grence, Andrea
author
Pacheco, Ana María
author
Marcos-Jiménez, Ana
author
Quiroga, Borja
author
Morado, Marta
author
Monroy, Francisco
author
Muñoz-Calleja, Cecilia
author
Sánchez-Madrid, Francisco
author
Urrutia, Andrés A
author
Aragonés, Julián
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
2023-11-02T10:07:52Z
2023-11-02T10:07:52Z
2023-01-20
iScience. 2022 Dec 5;26(1):105739.
http://hdl.handle.net/20.500.12105/16617
36582828
10.1016/j.isci.2022.105739
2589-0042
iScience
Inhibition of the heterodimeric amino acid carrier SLC7A5/SLC3A2 (LAT1/CD98) has been widely studied in tumor biology but its role in physiological conditions remains largely unknown. Here we show that the SLC7A5/SLC3A2 heterodimer is constitutively present at different stages of erythroid differentiation but absent in mature erythrocytes. Administration of erythropoietin (EPO) further induces SLC7A5/SLC3A2 expression in circulating reticulocytes, as it also occurs in anemic conditions. Although Slc7a5 gene inactivation in the erythrocyte lineage does not compromise the total number of circulating red blood cells (RBCs), their size and hemoglobin content are significantly reduced accompanied by a diminished erythroblast mTORC1 activity. Furthermore circulating Slc7a5-deficient reticulocytes are characterized by lower transferrin receptor (CD71) expression as well as mitochondrial activity, suggesting a premature transition to mature RBCs. These data reveal that SLC7A5/SLC3A2 ensures adequate maturation of reticulocytes as well as the proper size and hemoglobin content of circulating RBCs.
eng
Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation.
journal article
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URL
https://repisalud.isciii.es/bitstreams/8d2b4d64-2e9f-4648-a888-5f7b6d20bd61/download
File
MD5
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application/pdf
Erythroid_iScience_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/1a7723e1-74b0-4b2b-b013-fa908f7bca9c/download
File
MD5
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text/plain
Erythroid_iScience_2022.pdf.txt