2026-04-18T16:00:32Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/166172024-09-27T09:58:26Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Bouthelier, Antonio author Fernández-Arroyo, Lucía author Mesa-Ciller, Claudia author Cibrian, Danay author Martín-Cófreces, Noa Beatriz author Castillo-González, Raquel author Calero, Macarena author Herráez-Aguilar, Diego author Guajardo-Grence, Andrea author Pacheco, Ana María author Marcos-Jiménez, Ana author Quiroga, Borja author Morado, Marta author Monroy, Francisco author Muñoz-Calleja, Cecilia author Sánchez-Madrid, Francisco author Urrutia, Andrés A author Aragonés, Julián author 2023-01-20 Inhibition of the heterodimeric amino acid carrier SLC7A5/SLC3A2 (LAT1/CD98) has been widely studied in tumor biology but its role in physiological conditions remains largely unknown. Here we show that the SLC7A5/SLC3A2 heterodimer is constitutively present at different stages of erythroid differentiation but absent in mature erythrocytes. Administration of erythropoietin (EPO) further induces SLC7A5/SLC3A2 expression in circulating reticulocytes, as it also occurs in anemic conditions. Although Slc7a5 gene inactivation in the erythrocyte lineage does not compromise the total number of circulating red blood cells (RBCs), their size and hemoglobin content are significantly reduced accompanied by a diminished erythroblast mTORC1 activity. Furthermore circulating Slc7a5-deficient reticulocytes are characterized by lower transferrin receptor (CD71) expression as well as mitochondrial activity, suggesting a premature transition to mature RBCs. These data reveal that SLC7A5/SLC3A2 ensures adequate maturation of reticulocytes as well as the proper size and hemoglobin content of circulating RBCs. iScience. 2022 Dec 5;26(1):105739. http://hdl.handle.net/20.500.12105/16617 36582828 10.1016/j.isci.2022.105739 2589-0042 iScience Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation.