2026-06-14T03:33:04Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/166012024-09-27T20:41:42Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Sachse, Martin
author
Fernández de Castro, Isabel
author
Tenorio, Raquel
author
Risco, Cristina
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Agencia Estatal de Investigación (España)
funder
Consejo Superior de Investigaciones Científicas (España)
2023-10-23T10:04:26Z
2023-10-23T10:04:26Z
2024
Mol Microbiol. 2024 Apr;121(4):688-695.
10.1111/mmi.15182
1365-2958
Molecular microbiology
37864540
http://hdl.handle.net/20.500.12105/16601
Transmission electron microscopy (TEM) has been essential to study virus-cell interactions. The architecture of viral replication factories, the principles of virus assembly and the components of virus egress pathways are known thanks to the contribution of TEM methods. Specially, when studying viruses in cells, methodologies for labeling proteins and other macromolecules are important tools to correlate morphology with function. In this review, we present the most widely used labeling method for TEM, immunogold, together with a lesser known technique, metal-tagging transmission electron microscopy (METTEM) and how they can contribute to study viral infections. Immunogold uses the power of antibodies and electron dense, colloidal gold particles while METTEM uses metallothionein (MT), a metal-binding protein as a clonable tag. MT molecules build gold nano-clusters inside cells when these are incubated with gold salts. We describe the necessary controls to confirm that signals are specific, the advantages and limitations of both methods, and show some examples of immunogold and METTEM of cells infected with viruses.
eng
Electron microscopy
Immunogold
Labeling
METTEM
Tomography
Virus
Molecular mapping of virus-infected cells with immunogold and metal-tagging transmission electron microscopy
review article
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URL
https://repisalud.isciii.es/bitstreams/8ee5990e-f9be-4f77-bd7e-c01799d62463/download
File
MD5
171fc06f5542a9f18a68038ebb02adc0
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application/pdf
MolecularMappingVirus-infectedCells_2023.pdf
URL
https://repisalud.isciii.es/bitstreams/e5a92ec3-0a01-4ad0-a495-2be955e656e6/download
File
MD5
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text/plain
MolecularMappingVirus-infectedCells_2023.pdf.txt