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oai:repisalud.isciii.es:20.500.12105/165672024-05-27T19:32:34Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Maier, Alexander author Toner, Yohana C author Munitz, Jazz author Sullivan, Nathaniel A T author Sakurai, Ken author Meerwaldt, Anu E author Brechbühl, Eliane E S author Prévot, Geoffrey author van Elsas, Yuri author Maas, Rianne J F author Ranzenigo, Anna author Soultanidis, Georgios author Rashidian, Mohammad author Pérez-Medina, Carlos author Heo, Gyu Seong author Gropler, Robert J author Liu, Yongjian author Reiner, Thomas author Nahrendorf, Matthias author Swirski, Filip K author Strijkers, Gustav J author Teunissen, Abraham J P author Calcagno, Claudia author Fayad, Zahi A author Mulder, Willem J M author van Leent, Mandy M T author 2023-07 In the past 2 decades, research on atherosclerotic cardiovascular disease has uncovered inflammation to be a key driver of the pathophysiological process. A pressing need therefore exists to quantitatively and longitudinally probe inflammation, in preclinical models and in cardiovascular disease patients, ideally using non-invasive methods and at multiple levels. Here, we developed and employed in vivo multiparametric imaging approaches to investigate the immune response following myocardial infarction. The myocardial infarction models encompassed either transient or permanent left anterior descending coronary artery occlusion in C57BL/6 and Apoe-/-mice. We performed nanotracer-based fluorine magnetic resonance imaging and positron emission tomography (PET) imaging using a CD11b-specific nanobody and a C-C motif chemokine receptor 2-binding probe. We found that immune cell influx in the infarct was more pronounced in the permanent occlusion model. Further, using 18F-fluorothymidine and 18F-fluorodeoxyglucose PET, we detected increased hematopoietic activity after myocardial infarction, with no difference between the models. Finally, we observed persistent systemic inflammation and exacerbated atherosclerosis in Apoe-/- mice, regardless of which infarction model was used. Taken together, we showed the strengths and capabilities of multiparametric imaging in detecting inflammatory activity in cardiovascular disease, which augments the development of clinical readouts. JACC Basic Transl Sci. 2023 Apr 5;8(7):801-816. http://hdl.handle.net/20.500.12105/16567 37547068 10.1016/j.jacbts.2022.12.014 2452-302X JACC. Basic to translational science Multiparametric Immunoimaging Maps Inflammatory Signatures in Murine Myocardial Infarction Models.