2026-06-14T00:35:10Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/165662024-09-27T09:59:06Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Rodriguez-Galan, Ana author Dosil, Sara G author Hrčková, Anna author Fernandez-Messina, Lola author Feketová, Zuzana author Pokorná, Julie author Fernandez-Delgado, Irene author Camafeita, Emilio author Gómez, Manuel José author Ramírez-Huesca, Marta author Gutierrez-Vazquez, Cristina author Sanchez-Cabo, Fatima author Vazquez, Jesus author Vaňáčová, Štěpánka author Sánchez-Madrid, Francisco author 2023-08-30 ISG20L2, a 3' to 5' exoribonuclease previously associated with ribosome biogenesis, is identified here in activated T cells as an enzyme with a preferential affinity for uridylated miRNA substrates. This enzyme is upregulated in T lymphocytes upon TCR and IFN type I stimulation and appears to be involved in regulating T cell function. ISG20L2 silencing leads to an increased basal expression of CD69 and induces greater IL2 secretion. However, ISG20L2 absence impairs CD25 upregulation, CD3 synaptic accumulation and MTOC translocation towards the antigen-presenting cell during immune synapsis. Remarkably, ISG20L2 controls the expression of immunoregulatory molecules, such as AHR, NKG2D, CTLA-4, CD137, TIM-3, PD-L1 or PD-1, which show increased levels in ISG20L2 knockout T cells. The dysregulation observed in these key molecules for T cell responses support a role for this exonuclease as a novel RNA-based regulator of T cell function. Cell Mol Life Sci. 2023 Aug 30;80(9):273. 10.1007/s00018-023-04925-2 1420-9071 Cellular and molecular life sciences : CMLS 37646974 http://hdl.handle.net/20.500.12105/16566 ISG20L2: an RNA nuclease regulating T cell activation.