2026-07-21T20:40:48Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/165582024-09-27T08:05:30Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Petzold, Tobias author Zhang, Zhe author Ballesteros, Ivan author Saleh, Inas author Polzin, Amin author Thienel, Manuela author Liu, Lulu author Ul Ain, Qurrat author Ehreiser, Vincent author Weber, Christian author Kilani, Badr author Mertsch, Pontus author Götschke, Jeremias author Cremer, Sophie author Fu, Wenwen author Lorenz, Michael author Ishikawa-Ankerhold, Hellen author Raatz, Elisabeth author El-Nemr, Shaza author Görlach, Agnes author Marhuenda, Esther author Stark, Konstantin author Pircher, Joachim author Stegner, David author Gieger, Christian author Schmidt-Supprian, Marc author Gaertner, Florian author Almendros, Isaac author Kelm, Malte author Schulz, Christian author Hidalgo, Andres author Massberg, Steffen author 2022-12-13 Intravascular neutrophils and platelets collaborate in maintaining host integrity, but their interaction can also trigger thrombotic complications. We report here that cooperation between neutrophil and platelet lineages extends to the earliest stages of platelet formation by megakaryocytes in the bone marrow. Using intravital microscopy, we show that neutrophils "plucked" intravascular megakaryocyte extensions, termed proplatelets, to control platelet production. Following CXCR4-CXCL12-dependent migration towards perisinusoidal megakaryocytes, plucking neutrophils actively pulled on proplatelets and triggered myosin light chain and extracellular-signal-regulated kinase activation through reactive oxygen species. By these mechanisms, neutrophils accelerate proplatelet growth and facilitate continuous release of platelets in steady state. Following myocardial infarction, plucking neutrophils drove excessive release of young, reticulated platelets and boosted the risk of recurrent ischemia. Ablation of neutrophil plucking normalized thrombopoiesis and reduced recurrent thrombosis after myocardial infarction and thrombus burden in venous thrombosis. We establish neutrophil plucking as a target to reduce thromboischemic events. Immunity. 2022 Dec 13;55(12):2285-2299.e7. 10.1016/j.immuni.2022.10.001 1097-4180 Immunity 36272416 http://hdl.handle.net/20.500.12105/16558 Neutrophil "plucking" on megakaryocytes drives platelet production and boosts cardiovascular disease.