2026-07-23T17:11:50Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/165342024-10-31T11:51:22Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Juste, Ramón A
author
Blanco-Vázquez, Cristina
author
Barral, Marta
author
Prieto, José Miguel
author
Varela-Castro, Lucía
author
Lesellier, Sandrine
author
Dave, Dipesh
author
Sevilla, Iker A
author
Martín Ezquerra, Ana Belén
author
Adriaensen, Hans
author
Herrero-García, Gloria
author
Garrido, Joseba M
author
Casais, Rosa
author
Balseiro, Ana
funder
Plan Nacional de I+D+i (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Agencia Estatal de Investigación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Gobierno del Principado de Asturias (España)
funder
Unión Europea. Comisión Europea. H2020
funder
Junta de Castilla y León (España)
2023-09-29T10:24:19Z
2023-09-29T10:24:19Z
2023-09
Heliyon. 2023 Aug 21;9(9):e19349.
10.1016/j.heliyon.2023.e19349
2405-8440
Heliyon
37662827
http://hdl.handle.net/20.500.12105/16534
Badgers (Meles meles) are a major tuberculosis (TB) reservoir in Europe, with the potential to transmit infection to cattle. Here we assessed whether a recently described oral tuberculosis vaccine based on heat-inactivated Mycobacterium bovis (HIMB), delivered as edible baits, can protect badgers from infection. Eight badgers were given individually five baits, each one consisting of a ball of peanut butter, natural peanut and oat flakes including a dose of the vaccine containing 5 × 107 colony-forming units. In parallel, a control group of seven badgers did not receive the vaccine. One month and a half later a second dose of the vaccine was offered to the vaccinated group. Ninety-four days after the second dose, all badgers were challenged with M. bovis (103 colony-forming units per animal) delivered endobronchially to the right middle lung lobe. Clinical, immunological, pathological and bacteriological variables were measured throughout the whole study to assess the efficacy of the vaccine. Two vaccinated animals showed high bacterial load of M. bovis and worsening of pathological lesions of TB. Conversely, the other six vaccinated animals showed slight improvement in bacterial load and pathology with respect to the control group. These results suggest that delivering the TB vaccine via food bait can partially protect wild badger populations, although vaccination can lead to either protection or tolerization, likely depending on the animal's immune status and general condition at the time of vaccination. Further optimization of the vaccination trial/strategy is needed to reduce the rate of tolerization, such as altering vaccine dose, number of doses, type of bait, use of adjuvants or route of administration.
eng
Badger
Efficacy
HIMB
Mycobacterium bovis heat-inactivated bait oral vaccine
Tuberculosis
Efficacy of heat-inactivated Mycobacterium bovis vaccine delivered to European badgers (Meles meles) through edible bait
research article
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