2026-05-21T23:39:19Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/165142024-09-28T03:07:20Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Quinchia, Jennifer
author
Blázquez-García, Marina
author
Torrente-Rodríguez, Rebeca M
author
Ruiz-Valdepeñas Montiel, Víctor
author
Serafín, Verónica
author
Rejas-González, Raquel
author
Montero-Calle, Ana Maria
author
Orozco, Jahir
author
Pingarrón, José M
author
Barderas Manchado, Rodrigo
author
Campuzano, Susana
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Comunidad de Madrid (España)
funder
Ministerio de Educación, Cultura y Deporte (España)
funder
University of Antioquia (Colombia)
funder
Max Planck Society
2023-09-26T13:47:18Z
2023-09-26T13:47:18Z
2023-09-04
Talanta. 2023 Sep 4;267:125155.
10.1016/j.talanta.2023.125155
1873-3573
Talanta
37696234
http://hdl.handle.net/20.500.12105/16514
Detecting overexpression of cancer biomarkers is an excellent tool for diagnostic/prognostic and follow-up of patients with cancer or their response to treatment. This work illustrates the relevance of interrogating the levels of T-cell immunoglobulin and mucin domain 1 (TIM-1) protein as a diagnostic/prognostic biomarker of high-prevalence breast and lung cancers by using an amperometric disposable magnetic microparticles-assisted immunoplatform. The developed method integrates the inherent advantages of carboxylic acid-functionalized magnetic beads (HOOC-MBs) as pre-concentrator support and the amperometric transduction at screen-printed carbon electrodes (SPCEs). The immunoplatform involves a sandwich-type immunoassay assembled on HOOC-MBs through the specific capture/labeling of TIM-1 using capture antibodies and horseradish peroxidase (HRP)-conjugated biotinylated detection antibodies as biorecognition elements. The magnetic immunoconjugates were confined onto the working electrode (WE) surface of the SPCEs for amperometric detection using the hydroquinone/hydrogen peroxide/HRP (HQ/H2O2/HRP) redox system. The method allows the selective detection of TIM-1 protein over the 87-7500 pg mL-1 concentration range in only 45 min, with a limit of detection of 26 pg mL-1. The developed bioplatform was successfully applied to the analysis of breast and lung cancer cell extracts, providing the first quantitative results of the target glycoprotein in these types of samples.
eng
Amperometric detection
Breast and lung cancer
Immunoplatform
Magnetic particles
Screen-printed carbon electrode
TIM-1
Disposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion
research article
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DisposableElectrochemicalImmunoplatformShed_2023.pdf
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https://repisalud.isciii.es/bitstreams/3e8b6ead-a215-4b33-879e-bf2ed5165ef5/download
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text/plain
DisposableElectrochemicalImmunoplatformShed_2023.pdf.txt