2026-08-29T14:05:09Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/165142024-09-28T03:07:20Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617

00925njm 22002777a 4500 dc Quinchia, Jennifer author Blázquez-García, Marina author Torrente-Rodríguez, Rebeca M author Ruiz-Valdepeñas Montiel, Víctor author Serafín, Verónica author Rejas-González, Raquel author Montero-Calle, Ana Maria author Orozco, Jahir author Pingarrón, José M author Barderas Manchado, Rodrigo author Campuzano, Susana author 2023-09-04 Detecting overexpression of cancer biomarkers is an excellent tool for diagnostic/prognostic and follow-up of patients with cancer or their response to treatment. This work illustrates the relevance of interrogating the levels of T-cell immunoglobulin and mucin domain 1 (TIM-1) protein as a diagnostic/prognostic biomarker of high-prevalence breast and lung cancers by using an amperometric disposable magnetic microparticles-assisted immunoplatform. The developed method integrates the inherent advantages of carboxylic acid-functionalized magnetic beads (HOOC-MBs) as pre-concentrator support and the amperometric transduction at screen-printed carbon electrodes (SPCEs). The immunoplatform involves a sandwich-type immunoassay assembled on HOOC-MBs through the specific capture/labeling of TIM-1 using capture antibodies and horseradish peroxidase (HRP)-conjugated biotinylated detection antibodies as biorecognition elements. The magnetic immunoconjugates were confined onto the working electrode (WE) surface of the SPCEs for amperometric detection using the hydroquinone/hydrogen peroxide/HRP (HQ/H2O2/HRP) redox system. The method allows the selective detection of TIM-1 protein over the 87-7500 pg mL-1 concentration range in only 45 min, with a limit of detection of 26 pg mL-1. The developed bioplatform was successfully applied to the analysis of breast and lung cancer cell extracts, providing the first quantitative results of the target glycoprotein in these types of samples. Talanta. 2023 Sep 4;267:125155. 10.1016/j.talanta.2023.125155 1873-3573 Talanta 37696234 http://hdl.handle.net/20.500.12105/16514 Amperometric detection Breast and lung cancer Immunoplatform Magnetic particles Screen-printed carbon electrode TIM-1 Disposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion