2026-04-29T07:35:54Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/165132024-09-28T02:58:30Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Tejerina-Miranda, Sandra
author
Pedrero, María
author
Blázquez-García, Marina
author
Serafín, Verónica
author
Montero-Calle, Ana Maria
author
Garranzo-Asensio, Maria
author
Julio Reviejo, A
author
Pingarrón, José M
author
Barderas Manchado, Rodrigo
author
Campuzano, Susana
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Comunidad de Madrid (España)
funder
Ministerio de Educación, Cultura y Deporte (España)
2023-09-26T13:24:09Z
2023-09-26T13:24:09Z
2023-09-14
Bioelectrochemistry. 2023 Sep 14;155:108571.
http://hdl.handle.net/20.500.12105/16513
37717337
10.1016/j.bioelechem.2023.108571
1878-562X
Bioelectrochemistry (Amsterdam, Netherlands)
This work reports the first electrochemical bioplatform developed for the determination of human endostatin (HE), a biomarker with recognized antiangiogenic potential whose elevated circulating levels have also been associated with the development of aggressive cancers. The developed electroanalytical biotool combines the benefits of using magnetic microparticles for the implementation of sandwich immunoassays and amperometric transduction on disposable carbon electrodes. A limit of detection (LOD) of 34.1 pg mL-1 for HE standards and a selectivity suitable for its foray into the clinical oncology area, are demonstrated. The determination of HE in clinical samples such as lysates and secretomes of colorectal cancer (CRC) cells, plasma, and tissue samples from patients with CRC in different stages, has been faced with satisfactory results showing the ability for discriminating the metastatic capabilities of cells and for identifying and staging CRC patients. The developed bioplatform allows precise quantitative determinations, requiring minimal pre-treatments and sample amounts in only 75 min. In addition, due to the instrumentation and the type of substrates used in the detection step, the biotool is compatible with implementation in multiplexed and/or point-of-need devices, features in which this bioplatform is advantageous with respect to the enzyme linked immunosorbent assay (ELISA) or immunoblotting technologies.
eng
Angiogenesis
Colorectal cancer aggressiveness
Electrochemical bioplatform
Endostatin
Plasma
Tissue
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing
research article
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AngiogenesisInhibitorAggressivenessMarker_2023.pdf
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Supplementary_AngiogenesisInhibitorAggressivenessMarker_2023.pdf
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AngiogenesisInhibitorAggressivenessMarker_2023.pdf.txt
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https://repisalud.isciii.es/bitstreams/8eeeeaf2-3dfd-4087-9fae-19c55c74e0c4/download
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Supplementary_AngiogenesisInhibitorAggressivenessMarker_2023.pdf.txt